人类心脏发育候选基因ZNF569的生物信息学分析

Bininformatical Analysis on Heart Developmental Candidate Gene ZNF569

目的分析人类心脏发育候选基因ZNF569的生物信息学,对其功能研究提供指导意见。方法利用生物信息学网站及生物软件对人类心脏发育候选基因ZNF569进行生物信息学分析。结果ZNF569是一个新的人类KRAB/C2H2型锌指蛋白基因。此基因在基因组DNA上长约56.28 kb,定位于19q13.12。开放阅读框全长2061个碱基,含6个外显子,5个内含子,编码一个长686个氨基酸残基的蛋白质。该蛋白包含一个KRAB结构域和18个锌指结构域,进化分析表明各种真核生物中都存在ZNF569的同源蛋白,而ZNF569蛋白与鼠ZNF74蛋白的相似度达到94%,它们之间的蛋白质序列保守度更高。ZNF569本身18个锌指结构蛋白质序列也高度保守。结论ZNF569及其同源蛋白构成了一个在进化中高度保守的新的KRAB-ZF基因,而它的功能尚未见报道。

Aim To analyse bininformaties on heart developmental candidate gene ZNF569 providing guiding opinion for functional study of ZNF569. Methods Bininformatieal analysis on ZNF569 was made by utilizing Bininformatieal Web and soft ware. Results Protein containing KRAB/C2H2 is an important transcriptional factor. Alignment between the eDNA sequence and human genome indicates that ZNF569 is identical on chromosome 19q13.12, spanning approximately 56.28 kb in reverse manner on the genome and organized into 6 exons ZNF569 encodes a putative protein of 686 amino acids. The predicted amino acid sequenee of ZNF569 contains an N-terminal Krtippel-associated box （ KRAB ） domain, and a series of 18 C2H2 zinc finger motifs that extend to the end of the protein sequence. A search of published DNA databases for sequences similar to that of ZNF569 demonstrated that they had varying degrees of similarity to a number of previously identified C2H2-type zine-fingers protein. Sequence analysis and database comparison indieate that the predicted protein contains 18 different C2H2 zinc-finger domains in tandem arrays, characteristic of transcription factor proteins of this family. Conclusion A search of analyzed evolutionary relationship among different zinc-finger proteins with phylogenetie tree analysis show that ZNF569 is one of the conserved proteins during evolution.