罗格列酮通过上调脂肪脂联素及其受体的表达改善去卵巢大鼠胰岛素抵抗

Rosiglitazone Improves Insulin Resistance by Up-Regulating the Expressions of Adiponectin and Its Receptors in the Adipose Tissue in Ovariectomized Rats

目的研究罗格列酮对去卵巢大鼠胰岛素抵抗和脂肪中脂联素及其受体表达的影响,为噻唑烷二酮类药物用于防治绝经后2型糖尿病提供实验依据。方法36只SD雌性大鼠,随机分为假手术组、去卵巢组和去卵巢+罗格列酮组;检测各组大鼠体重、血压和胰岛素敏感指数的变化。测量内脏脂肪重量,计算体脂含量;逆转录聚合酶链反应检测内脏脂肪中脂联素、脂联素受体的表达。结果与假手术组相比,去卵巢组大鼠内脏脂肪含量和血压显著增加,胰岛素敏感指数显著降低,内脏脂肪中脂联素、脂联素受体表达显著降低;罗格列酮逆转了上述变化。结论去卵巢大鼠胰岛素抵抗可能与脂肪脂联素及其受体的表达下调有关;罗格列酮能够上调脂肪脂联素及其受体的表达而改善去卵巢大鼠胰岛素抵抗。

Aim To investigate the effects of rosiglitazone on insulin resistance and the expression of adiponectin and its receptors in the adipose tissue in ovariectomized rats. Methods Thirty-six female Sprague-Dawley rats were randomly divided into three groups ： the sham control group, the ovariectomized group and the ovariectomized ＋ rosiglitazone group. The changes of insulin sensitive index （ISI）, blood pressure were measured. The content of visceral adipose depots was observed. The expression of adiponectin and its receptors （ AdipoR1 and AdipoR2 ） in the visceral adipose tissue were analyzed by reverse transcription polymerase chain reaction （RT-PCR）. Results Compared with the sham control group, the blood pressure and the content of visceral adipose depots were significantly increased, ISI was significantly decreased, and the expressions of adiponectin and AdipoR1/R2 in visceral adipose were significantly decreased in the ovariectomized rats. Compared with the ovariectomized group, these effects were reversed by rosiglitazone in the ovariectomized ＋ rosiglitazone group. Conclusion Rosiglitazone improved insulin resistance by up-regnlating the expression of adiponectin and its receptors in the adipose tissue in ovariectomized rats.