康复训练对血管性痴呆大鼠学习记忆能力及海马区神经细胞黏附因子表达的影响

The effects of rehabilitation on learning,memory and expression of NCAM in rats with vascular dementia

目的 探讨康复训练对血管性痴呆（VD）大鼠学习记忆的功能恢复及海马区神经细胞黏附因子（NCAM）表达的影响.方法 选择SD雌性大鼠45只,随机分为康复组20只、制动组20只和假手术组5只,采用双侧颈总动脉反复缺血再灌注加降血压法制作VD大鼠模型.3组分别于术后第27,28天以水迷宫试验评价大鼠学习记忆能力,采用核糖核酸逆转录聚合酶链反应（RT-PCR）法检测不同时间点海马区NCAM的表达.结果 水迷宫试验结果 显示,康复组学习记忆能力优于制动组,康复组NCAM在海马区的表达在术后第1天达到高峰,在第7,14天时间点康复组较制动组和假手术组均有明显增加.结论 康复训练可改善VD大鼠空间学习记忆能力,其分子机制可能与其海马上NCAM的表达水平的增高有关.

Objective To investigate the effect of rehabilitation training on learning and memory ability and the expression of neural cell adhesion molecule（NCAM）in rats modeling vascular dementia.Methods Foay-five female Sprague-Dawley rats were randomly assigned to a rehabilitation group（20 rats）,an immobilization group（20 rats）,or a sham-operation group（5 rats）.The experimental vascular dementia model was established by repeatedly clipping the common carotid artery to induce repetitive isehemia-reperfusion,and by reducing blood pressure with intra-abdominal injection of sodium nitroprusside.The rats＇ learning and memory were tested on the 27th and 28th days after the operation using a water-maze step-down avoidance test.A RT-PCR technique was used to detect NCAM expression around the hippocampal area at different times after the operation.Results The rehabilitation group rats showed significantly better learning and memory ability than those in the immobilization group.NCAM was also more strongly expressed in their hippocampi than in those of the immobilization group and sham-operation group.Conclusion Rehabilitation can accelerate recovery of learning and memory ability in rats,and the mechanism possibly is related to the increase of NCAM expression in the hippocampus.