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减重步行激活SCI大鼠腰膨大前角神经元效应的激光共聚焦镜检研究 被引量：1

An experimental study of neuronal activation through body-weight-supposed treadmill training after spinal cord injury using laser confocal microscopy
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摘要 目的 探讨减重平板步行训练（BWSTT）促进脊髓损伤（SCI）功能恢复的脊髓可塑性机制.方法 将84只成年雌性Wistar大鼠随意分为假手术组（Sham组,n=18）、胸髓横断模型组（SCI组,n=33）和减重平板步行训练组（BWSTT组,n=33）,每组大鼠再随机分为7,15,45 d 3个亚组.建立大鼠胸髓完全横断模型,观察BWSTT对SCI大鼠后肢运动功能的影响.采用激光共聚焦镜检免疫荧光双标技术.研究SCI大鼠腰髓前角腹内侧区神经元酪氨酸蛋白激酶受体（EphA4）、囊泡膜谷氨酸转运体2（VGluT2）的表达,以及两者双标免疫阳性细胞比率的变化.结果 BWSTT组大鼠的后肢运动功能较SCI组大鼠明显改善.胸髓横断大鼠BWSTT后,腰髓前角腹内侧区EphA4和VGIuT2的表达以及EphA4/VGhT2双标免疫阳性细胞比率均显著增加.结论 BWSTT通过增强胸髓横断大鼠腰髓前角神经元EphA4/VGIuT2的表达,促进后肢运动功能的恢复. Objective To investigate the effects of locomotor training on improving locomotor function after spinal cord injury（SCI）and the mechanism of spinal cord plasticity.Methods A model of complete thoracic cord transection was established using 84 adult female rats divided into sham,SCI and treadmill training（BWSTT） groups.The hind limb locomotor function of all the rats was evaluated.The fluorescence intensities due to （EphA4）,vesicular glutamate transporter 2（VGluT2）and EphA4/VGluT2 double-positive neurons in the ventromedial area of the anterior horn of the lumbar COrd were detected using immunofluorescence double labeling and laser confocal microscopy.Results The rats in the BWSTT group showed better functional recovery in their hind limbs than those in the SCI group.BWSTT was correlated with markedly increased EphA4.VGIuT2 and EphA4/VGluT2 intensities in the ventromedial area.Conclusion BWSTT improves hind limb locomotor function in rats with thoracic cord transections by elevating the expression of EphA4/VGluT2,promoting neuronal plasticity in the lumbar anterior horn.
作者 张缨 纪树荣 周红俊 范晓华 刘根林 郑樱 郝春霞 王一吉
机构地区 中国康复研究中心北京博爱医院脊髓康复科 山东省立医院康复科
出处 《中华物理医学与康复杂志》 CAS CSCD 北大核心 2009年第6期366-369,共4页 Chinese Journal of Physical Medicine and Rehabilitation
关键词 脊髓损伤 减重平板步行训练 酪氨酸蛋白激酶受体 囊泡膜谷氨酸转运体 脊髓可 塑性 Spinal cord injury Body-weight-supported treadmill training EphA4： Vesicular glutamate transporter 2 Spinal plasticity
分类号 R651.2 [医药卫生—外科学]
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参考文献18

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共引文献7

同被引文献28

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中华物理医学与康复杂志
2009年 第6期

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