电针配合神经生长因子对脑缺血大鼠学习记忆能力的影响

The effect of electro-acupuncture in combination with nerve growth factor on learning and memory in rats with cerebral ischemia

目的 观察电针配合外源性神经生长因子（NGF）对脑缺血大鼠学习记忆能力的影响.方法 将Spragne-Dawley大鼠40只按随机数字表分配法随机分为假手术组、模型组、NGF组、电针组和NGF＋电针组,每组大鼠8只.模型组、NGF组、电针组和NGF＋电针组用脑缺血-再灌注方法 建立学习记忆障碍大鼠动物模型,4组大鼠血脑屏障稳定后,NGF组和NGF＋电针组于尾静脉注射NGF（10μg/kg体重,每日1次,连续15 d）,电针组和NGF＋电针组同时进行电针治疗（取哑门、百会穴,频率100 Hz,输出电流2 mA,每日1次,每次20 min,连续15 d）,疗程结束后采用Morris水迷宫检测大鼠空间学习记忆能力的变化.结果 模型组、NGF组、电针组和NGF＋电针组大鼠造模后均出现明显的学习记忆障碍,与假手术组比较差异有统计学意义（P＜0.05）.各组治疗15 d后,NGF组、模型组和电针组之间,大鼠学习记忆能力无明显差异（P＞0.05）;NGF＋电针组大鼠学习记忆能力明显提高,与模型组、电针组、NGF组比较,差异有统计学意义（P＜0.01）.结论 电针配合神经生长因子对学习记忆障碍大鼠的学习能力具明显的改善作用.

Objective To observe the effect of nerve growth factor（NGF）and electro-acupuncture（EA） on learning and memory in rats with cerebral ischemia.Methods An experimental model of cerebral ischemiareperfusion was established in 40 rats,who were then randomly divided into a model group,a sham operated group,a NGF group,an acupuncture group and a NGF plus acupuncture group.Three weeks were allowed for the recovery of the blood-brain barrier lesion caused by ischemia-reperfusion before intravenous injection of NGF（10μg/kg,once daily for 15 days）via the vena caudalis.In the two acupuncture groups,this was administered in combination with EA at the Baihui（DU20）and Yamen（DU15）acupoints（frequency 100 Hz,2 mA,20 min/time,once daily for 15 days）.Morris＇ water maze test was used to assess any changes in spatial learning and memory abilities.Results The ischemic rats showed significant learning and memory disorders compared with the sham-operated group.In the NGF group,the rats＇ learning and memory abilities did not impmve significantly compared with the model group and the EA group.Significantly better learning was observed in the NGF＋EA group compared with the model group,the EA group and the NGF group.Conclusion NGF combined with EA can improve the learning and memory abilities of rats with learning and memory dysfunction caused by cerebral ischemia-repedusion.