微卫星标记在大菱鲆(Scophthalmus maximus L.)家系系谱印证中的应用研究

The application of microsatellite markers for genealogical identification in selective breeding program of turbot(Scophthalmus maximus L.)

用8个微卫星标记对大菱鲆7个人工选育家系进行了系谱鉴别和遗传多样性研究。8个微卫星位点的平均多态信息含量为0.6721,平均杂合度为0.7206。8个微卫星位点在7个家系分析中,共检测到40个等位基因,每个位点的等位基因数在38个之间,每个位点平均有5个等位基因。根据已知亲本及子代基因型,成功的推断出了7个家系中缺失亲本的基因型。在双亲未知的情况下8个微卫星位点累积排除概率是97.07%,已知单亲时累积排除概率达99.63%。用UPG-MA法对7个家系的137个子代个体进行了聚类分析,90.5%同一家系的子代个体能完全聚类到一起,分类结果与系谱来源基本一致。微卫星标记可以为大菱鲆混养家系进行亲权鉴定和系谱构建提供技术支持。

Eight microsatellite markers were used to estimate the kinship and the genetic diversity of seven turbot （Scophthalmus maximus L. ） families. The average polymorphic information content （PIC） and the average heterozygosity were 0. 6721 and 0. 7606, respectively. Totally forty alleles were detected at eight microsatellite loci in seven full-and half-sib families of turbot. The number of the alleles at each locus ranged from three to eight. Based on the genotypes of either known parent or none of them and offspring, all the absent parental genotypes of the seven families were deduced. According to the unique alleles among the families, each family could be distinguished from the others. Cervus3.0 analysis showed that the com- bined exclusion probability was 97.07% if parents were both unknown and the combined exclusion probability was 99.63 % when only one of the parental genotype was known. A dendrogram was constructed using UPGMA based on that 90. 5%of the indi ded with the source of logical identification of genetic similarity matrix. The UPGAM dendrogram of the 137 individuals indicated viduals from each family could be fully clustered and the classification results accorgenealogy. The results showed that microsatellite marker is useful tool for genea- turbot.