电针对抑郁大鼠行为学、海马生长抑素及其mRNA表达的影响

Influence of Electroacupuncture on Hippocampal Somatostatin(SS) and SS mRNA Expression in Chronic Stress-induced Depression Rats

目的:观察电针对抑郁大鼠行为学、海马生长抑素(somatostatin,SS)及SS mRNA表达的影响,探讨电针治疗抑郁症的可能机制。方法:30只健康Wistar大鼠,随机分为正常组、模型组和电针组,每组10只。慢性应激法刺激造抑郁大鼠模型,电针"百会""三阴交",每日1次,每次20 min,连续14 d。使用开野试验和糖水试验检测大鼠行为学变化,使用免疫组化法和逆转录多聚酶链反应法测定SS及SS mRNA的表达。结果:造模后模型组大鼠水平和垂直运动次数、糖水摄入量与正常组比较均降低(P<0.01),治疗后电针组的水平、垂直运动次数较治疗前及模型组均有升高(P<0.01)。模型组与正常组相比,SS、SS mRNA表达均降低(P<0.05);电针组与模型组比较,SS、SS mRNA表达均升高(P<0.05)。结论:电针可明显改善抑郁大鼠行为学,提高抑郁大鼠海马SS及SS mRNA的表达,电针的抗抑郁作用可能与之有关。

Objective To study the underlying mechanism of electroacupuncture （EA） in relieving depression. Methods A total of 30 Wistar rats were randomly divided into normal control group （n = 10）, model group （n = 10） and EA group in= 10）. Depression model was established by lonely raising and chronic unpredictable mild stress for 21 days. EA （2 Hz, 1 mA） was applied to ＂Baihui＂ （GV 20） and ＂Sanyinjiao＂ （SP 6） for 20 min, once daily for 14 days. The expression （option density） of hippocampal somatostatin （SS） and SS mRNA was detected by using immunohistochemistry and reversed transcription-polymerase chain reaction （RT-PCR） techniques respectively, Results Compared to normal control group, the crossing movement and rearing movement times of open-field test, and sucrose consumption in model group decreased significantly （P〈0.01 ）, and hippocampal SS and SS mRNA expressions were downregulated remarkably （P 〈 0.05）. Compared to model group, the crossing movement and rearing movement times in EA group increased considerably （P〈0.01）, and hippocampal SS and SS mRNA expressions were upregulated remarkably （P〈0.05）. No significant difference was found between EA and normal control groups in SS and SS mRNA expression. Conclusion EA can effectively improve depression rats＇ behavior activity and upregulate hippocampal SS and SS mRNA expression.