电针“内关”对室性心动过速大鼠心率及血浆儿茶酚胺含量的影响

Effect of Electroacupuncture of "Neiguan"(PC 6) on Heart Rate and Plasma Catecholamine Contents in Ventricular Tachycardia Rats

目的:探讨电针"内关"调节室性心动过速(VT)大鼠心率的作用机制。方法:SD大鼠50只,随机分为正常组、假手术组、模型组、电针内关组、电针列缺组,每组10只。采用股静脉注射氯化铯(CsCl)的方法建立大鼠VT模型,并连续观测心电图,记录心率变化。电针内关组和电针列缺组在造模成功后分别电针"内关"穴和"列缺"穴5 min。采用荧光法检测各组大鼠血浆中去甲肾上腺素(NE)、肾上腺素(E)的含量。结果:模型组、电针内关组及电针列缺组大鼠在注射CsCl造模后心率均显著增快(P<0.01);模型组与电针列缺组在整个观察期内心率未见恢复;电针内关组在电针治疗5 min后心率明显减慢,与模型组及电针列缺组比较差异均有统计学意义(P<0.01)。5组大鼠血浆NE、E含量比较,模型组较正常组、假手术组均显著升高(P<0.01),而电针内关组较模型组和电针列缺组均显著降低(P<0.01)。结论:电针内关可能通过抑制交感-肾上腺髓质系统活性,减少儿茶酚胺的释放,调整心率。

Objective To observe the effect of electroacupuncture （EA） of ＂Neiguan＂ （PC 6） on heart rate （HR） and plasma catecholamine （CA） levels in ventricular tachycardia （VT） rats. Methods A total of 50 SD rats were randomly divided into normal control, sham-operation （sham）, model, EA-PC 6 and EA-LU 7 groups, with 10 cases in each. VT model was established by intravenous injection of cesium chloride （CsCI） via femoral vein. Electrocardiogram （ECG） was recorded. EA （2 Hz/15 Hz, 1 mA） was separately applied to bilateral ＂Neiguan＂ （PC 6） and ＂Lieque＂ （LU 7） for 5 minutes, followed by collecting blood samples for detecting plasma norepinephrine （NE） and epinephrine （E） levels with fluorometric method. Results In comparison with normal control group, plasma NE and E contents in model group increased apparently （ P〈0.01 ）. Compared with model group, HR and plasma NE and E contents of EA-PC 6 group reduced significantly （ P〈0.01 ）, while those of EA-LU 7 had no apparent change （P〉0.05）. Conclusion EA of ＂Neiguan＂ （PC 6） can downregulate heart rate and plasma CA level in VT rats, which may contribute to its effect in relieving tachycardia.