前列腺癌细胞和组织中Axl及其配体蛋白的表达与临床意义

Expression of receptor tyrosine kinase Axl and its ligand in prostate cancer and its clinical significance

目的:探讨受体酪氨酸激酶Axl及其配体Gas6蛋白在前列腺癌细胞系和前列腺癌组织中的表达及其与前列腺癌病理特征的关系。方法:收集自2007年-2008年在第二军医大学长海医院泌尿外科接受手术或病理穿刺的前列腺癌组织标本45例和良性前列腺增生组织标本32例。Western blotting分析前列腺癌细胞系LNCaP、PC-3及DU-145中Axl及其配体Gas6的表达;免疫组化方法检测前列腺癌及良性前列腺增生组织中Axl及其配体Gas6的表达。结果:Western blotting检测结果显示,高侵袭性前列腺癌细胞DU-145和PC-3中Axl的表达显著强于低侵袭性细胞LNCaP,Gas6的表达在3个细胞系中无明显差异。免疫组化结果显示,前列腺癌组织中Axl蛋白强阳性表达显著高于良性前列腺增生组织(68.9%vs21.9%,P<0.01);前者Gas6蛋白的表达也显著高于后者(53.3%vs15.6%,P<0.01)。前列腺癌组织中,Axl和Gas6蛋白的强阳性表达与Gleason评分及远处转移密切相关(P<0.05)。结论:前列腺癌组织中Axl和Gas6高表达,该两者的表达与前列腺癌恶性程度及肿瘤转移密切相关。

Objective： To examine the expression of receptor tyrosine kinase（RTK） Axl and its ligand Gas6 （ growth arrest-specific gene 6） protein in prostate cancer （PCa） tissues and their relation with pathological characteristics of PCa. Methods： Forty-five PCa tissues and 32 benign prostatic hyperplasia tissues （2007 to 2008, Changhai Hospital） were obtained during surgery or kidney puncture. Expression of Axl and Gas6 protein in PCa cell lines LNCaP, PC-3 and DU-145 was analyzed by Western blotting. Expression of Axl and Gas6 protein in PCa tissues or benign prostatic hyperplasia tissues was examined by immunohistochemisty. Results： Axl expression was higher in highly invasive DU-145 and PCo3 cells than that in lowly invasive LNCaP cells. Expression of Gas6 in DU-145, PC-3 and LNCaP cells was similar. Expression of Axl and Gas6 protein in PCa tissues was significantly higher than that in benign prostatic hyperplasia tissues as detected by immunohistochemisty（68.9% vs 21.9% ; 53.3% vs 15.6% , all P 〈0.01 ）. Expression of Axl and Gas6 protein in PCa tissues was closely correlated with Gleason scores and metastasis of PCa （P 〈 0.05 ）. Conclusion： Expression of Axl and Gas6 in PCa is closely correlated with the malignancy degree and metastasis of PCa.