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小神经胶质细胞移植对脑梗死的治疗作用 被引量：2

Effects of microglia transplantation on treating cerebral infarction
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摘要 目的:探讨小神经胶质细胞(MG)移植对脑梗死的治疗作用及其机制。方法:采用光化学局灶性脑梗死大鼠动物模型,运用组织化学、免疫组织化学方法研究经锁骨下动脉移植入MG对梗死周边区的影响。并通过免疫荧光双标和Western blotting检测MG中神经生长因子(NGF)和白细胞介素-10(IL-10)的表达。结果:脑梗死灶的周围发现大量FITC绿色荧光标记的移植而来的MG;MG移植明显减小脑梗死灶体积、减少神经元凋亡;移植而来的MG中NGF和IL-10的表达显著增加。结论:MG能够通过血脑屏障(BBB)并游走至损伤部位,并通过分泌NGF和IL-10对脑血栓脑梗死灶的周围区产生保护作用,提示MG移植可能用于脑血栓的治疗。 AIM： To explore the regulating mechanism of microglia transplantation in treating cerebral infarction. METHODS： Focal cerebral infarction model was established by photochemistry. The histological and immunohistochemical methods were used to observe the effects of microglia transplantation on cerebral infarction. The expressions of nerve growth factor（NGF） and interleukin - 10（IL - 10） were detected by double fluorescent immunohistochemistry and Western blotting. RESULTS： Microglia marked with green fluorescence was observed in ischemic penumbra, indicating that microglia can be transplanted across the blood - brain barrier. Infarct volumes and death number of cells were significantly reduced compared to non - transplantation animals. The expressions of NGF and IL - 10 were markedly increased in microglia in transplantation group. CONCLUSION ： Microglia can be transplanted across the blood - brain barrier. The cells have protective effects in ischemic penumbra by secreting NGF and IL - 10, which might serve as therapeutical method for treating cerebral infarction.
作者 张剑 张敏
机构地区 绍兴文理学院医学院药理研究室 绍兴文理学院附属医院
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2009年第7期1331-1335,共5页 Chinese Journal of Pathophysiology
基金 国家自然科学基金资助项目(No.30800360) 国家人事部留学人员科技活动项目择优资助计划资助项目 浙江省自然科学基金资助项目(No.Y204364) 浙江省教育厅资助项目(No.20061164)
关键词 脑梗死 小神经胶质细胞 血脑屏障 神经生长因子 白细胞介素10 Brain infarction Microglia Blood - brain barrier Nerve growth factor Interleukin - 10
分类号 R332.81 [医药卫生—人体生理学]
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参考文献10

  • 1Suziki H,Imai F,Kanno T,et al.Preservation of neurotrophin expression in microglia that migrate into the gerbil's brain across the blood-brain barrier[J].Neuroaci Lett,2001,312(2):95-98.
  • 2Gabryel B,Trzeciak HI.Role of astrocytes in pathogenesis of ischemic brain injury[J].Neurotox Res,2001,3(2):205-221.
  • 3Yao H,Ibayashi S,Sugimori H,et al.Simplified model of krypton laser-induced thrombotic distal middle cerebral artery occlusion in spontaneously hypertensive rats[J].Stroke,1996,27(2):333-336.
  • 4黄秀艳,曾耀英,张彩,朱斌.人小胶质细胞的分离、纯化、特异分子表达与吞噬功能研究[J].中国病理生理杂志,2008,24(5):939-944. 被引量：8
  • 5Ahmad I,Das AV,James J,et al.Neural stem cells in the mannalian eye:types and regulation[J].Semin Cell Dev Biol,2004,15(1):53-62.
  • 6Suk K,Park JH,Lee WH,et al.Neuropeptide PACAP inhibits hypoxic activation of brain mierogli:a protective mechanism against microglial neurotoxicity in ischemia[J].Brain Res,2004,1026(1):151-156.
  • 7Yamasaki R,Zhang J,Koshiishi I,et al.Involvement of lysosomal storage-induced P38 MAP kinase activation in the overproduction of nitric oxide by microglia in cathepsin D-deficient mice[J].Mol Cell Neuresci,2007,35 (4):573-584.
  • 8Priller J,Flugel A,Wehner T,et al.Targeting gene-modified hematopoietic cells to the central nervous system:use of green fluorescent protein uncovers microglial engraftment[J].Nat Med,2001,7(12):1356-1361.
  • 9Shigeno T,Mima T,Takakura K,et al.Amelioration of delayed neuronal death in hippocampus by nerve growth factor[J].J Neurosci,1991,11(9):2914-2919.
  • 10Li HL,Kostulas N,Huang YM,et al.IL-17 and IFN-gamma mRNA expression is increased in the brain and systemically after pemmnent middle cerebral artery occlusion in the rat[J].J Neuroimmurnol,2001,116(1):5-14.

二级参考文献12

  • 1罗焕敏,邓惠,黄丰.RNA干扰对老年性痴呆症的治疗机制研究[J].中国病理生理杂志,2005,21(2):408-411. 被引量：3
  • 2Hanisch UK, Kettenmann H. Microglia: active sensor and versatile effector cells in the normal and pathologic brain [J]. Nat Neurosci, 2007, 10( 11 ) :1387 - 1394.
  • 3Garden GA, Moller T. Microglia biology in health and disease [J]. J Neuroimmue Pharmacol, 2006, 1(2):127- 137.
  • 4Block ML, Zecca L, Hong JS. Microglia - mediated neurotoxicity : uncovering the molecular mechanisms [ J ]. Nat Rev Neurosci, 2007, 8(1 ) :57 -69.
  • 5McCarthy KD, DeVellis J. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue [J]. J Cell Biol, 1980, 85(3) : 890 -902.
  • 6Cardona AE, Huang D, Sasse ME, et al. Isolation of murine microglial cells for RNA analysis or flow cytometry [ J ]. Nat Protoc, 2006, 1 (4) : 1947 - 1951.
  • 7Giuhan D, Baker TJ. Characterization of ameboid microglia isolated from developing mammalian, brain [ J ]. J Neurosci, 1986, 6 ( 8 ) : 2163 - 2178.
  • 8Giulian D, Li J, Barrel S, et al. Cell surface morphology identifies microglia as a distinct class of mononuclear phagocyte [J]. J Neurosci, 1995, 15(11):7712-7726.
  • 9Haynes SE, Hollopeter G, Yang G, et al. The P2Y12 receptor regulates microglial activation by extracellular nucleotides [J]. Nat Neurosci, 2006, 9(12) :1512- 1519.
  • 10Jack CS, Arbour N, Manusow J, et al. TLR signaling tailors innate immune responses in human microglia and astrocytes [J]. J Immunol, 2005, 175(7) :4320 -4330.

共引文献7

同被引文献17

  • 1余术宜,方云祥.小胶质细胞与脑缺血[J].神经疾病与精神卫生,2005,5(4):313-316. 被引量：1
  • 2Elkabes S, DiCicco-Bloom EM, Black IB. Brain microglia/ macro phages express neurotrophins that selectively regulate microglial pro-liferation and function[J]. J Neurosci, 1996, 16! 2 508-2 521.
  • 3McGeer PL, McGeer EG. The inflammatory response system of brain: implications for therapy of Alzheimer and other neu rodegenerative diseases[J]. Brain Res, 1995, 217195-218.
  • 4Dashdemberel Narantuya, Atsushi Nagai. Human Microglia Transplanted in Rat Focal Isehemia Brain Induce Neuroprotection and Behavioral Improvement[J]. PLoS One, 2010, 5(7): e11746.
  • 5Block ML, Zecca L, Hong JS. Microglia-mediated neuro toxicity: uncovering the molecular mechanisms[J]. Nat Rev Neu rosci, 2007, 8: 57-69.
  • 6Dirnagl U, Iadecola C, Moskowitz MA. Pathobiology of ischaemic stroke: an integrated view[J]. Trends Neurosci, 1999, 22:391-397.
  • 7Graeber MB,Streit WJ. Microglia:biology and pathology[ J]. Acta Neuropathol,2010 ; 119 ( 1 ) : 89-105.
  • 8Napoli I, Neumann H. Microglial clearance function in health and disease [J]. Neurosciencc,2009 ; 158 ( 3 ) : 1030-8.
  • 9Milligan ED, Watkins LR. Pathological and protective roles of glia in chronic pain[J]. Nat Rev Neurosci ,2009;10( 1 ) :23-36.
  • 10Haniseh UK, Kettenmann H. Microglia : active sensor and versatile effector cells in the normal and pathologic brain [J]. Nat Neurosci,2007; 10 ( 11 ) :1387-94.

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中国病理生理杂志
2009年 第7期

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