青枯菌拮抗菌2-Q-9的分子鉴定及抑菌相关基因的克隆

Identification of antagonistic bacteria 2-Q-9 against Ralstonia solanacearum and cloning function gene CodY

通过16SrDNA碱基序列的测定和同源性分析,鉴定青枯菌拮抗菌2-Q-9与桥石短芽孢杆菌(Brevibacillus choshinensis)亲缘关系最近,其16SrDNA序列提交GenBank,登录号为FJ613127.根据已测得的该拮抗菌外泌抗菌肽氨基酸序列中的一段设计简并引物,利用染色体步移法得到全长序列780bp,经测序和BLAST比对分析表明,该基因属于CodY家族,与已鉴定的转录因子抑制剂CodY(ZP_01171531)的同源性最高;表现在氨基酸水平的相似性为77%,其序列全长已提交GenBank,登录号为FJ613128.

Antagonistic bacteria 2-Q-9 against Ralstonia solanacearum indicated that the relationship between phylogenetic and Brevibacillus choshinensis was the closest based on the results of phylogenetic analysis of 16SrDNA sequence. The 16SrDNA sequence was registered at GenBank database with the accession number FJ613127. Based on one fragment of amino acid sequences of antibacterial peptides which secreted by Brevibacillus choshinensis strain 2-Q-9, degenerate primers were designed. A target fragment with a full length of 780 bp was amplified using genome walking. BLAST indicated that this sequence is similar to the sequence of transcriptional repressor CodY with amino acid sequence similarity 77%, so it was a member of CodY family. The full length sequence was registered at GenBank database with the accession number FJ613128.