期刊文献+

116例阳性血清中丙型肝炎病毒RNA定量检测及其抗体丙氨酸转氨酶结果分析 被引量:3

下载PDF
导出
摘要 我国一般人群丙型肝炎病毒(HCV)感染率约为3.2%(0.1%。10%),HCV感染严重危害人类的健康,早期发现HCV感染患者,可及时进行抗HCV治疗。抗-HCV抗体及HCV核糖核酸(HCV RNA)是诊断丙型肝炎的主要病原学指标。本文采用EIA法检测抗-HCV,用荧光定量聚合酶链反应(FQ—PCR)技术检测HCV RNA,同时用全自动生化分析仪检测丙氨酸转氨酶(ALT),对结果进行比较分析,探讨两种检测方法在临床诊断治疗中的意义。
作者 杨国华 曹燕
出处 《实用医技杂志》 2009年第8期648-649,共2页 Journal of Practical Medical Techniques
  • 相关文献

参考文献3

二级参考文献10

  • 1[1]Didomenico N,Link H,Konbel R,et al.COBAS Amplieor:fully automated RNA and DNA amplification and detection system for routine PCR[J].Clin Chem,1996,42(12):1915-1923.
  • 2[2]Maria M,Jordi G,Juan IE,et al.High throughout realtime reverse transcription-PCR quantitation of HCV RNA[J].J Chin Mierobiol,1999,379(4):327-332.
  • 3[4]Lee YS,Yoon SK,Chung ES,et al.The relationship of histologic activity to serum ALT.HCV genotype and HCV RNA titers in chronic hepatits C[J].J Korean Med Sci,2001,16(5):585-591.
  • 4[5]Sarrazin C.Highly sensitive hepatitis C virus RNA detection methods:molecular backgrounds and clinical significance[J].J Clin Virol,2002,25(3):523-529.
  • 5Wang TY,Kuo HT,Chen LG,et al.Use of polymerase chain reaction for early detection and management of heapatitis C virus infection after needlestick injury[J].Ann Clin Lab SciM,2002,32(2):137
  • 6Edward Doo MD. Epidemiology and immunopathogenesis of chronic hepatitis B virus infection[J] 2003,Current Hepatitis Reports(2):79~81
  • 7M. Esumi,T. Rikihisa,S. Nishimura,J. Goto,K. Mizuno,Y.-H. Zhou,T. Shikata. Experimental vaccine activities of recombinant E1 and E2 glycoproteins and hypervariable region 1 peptides of hepatitis C virus in chimpanzees[J] 1999,Archives of Virology(5):973~980
  • 8郑怀竞.献血者丙型肝炎病毒“窗口期”感染的筛查技术展望[J].中华肝脏病杂志,2002,10(2):159-160. 被引量:31
  • 9程钢,何蕴韶,周新宇,李虎.丙型肝炎病毒(HCV)荧光PCR(F-PCR)试剂盒的研制及与免疫学方法的比较[J].中国免疫学杂志,2002,18(7):464-468. 被引量:10
  • 10成军.丙型肝炎病毒致病的分子生物学机制[J].解放军医学杂志,2003,28(1):23-27. 被引量:41

共引文献55

同被引文献19

  • 1杨振.丙型肝炎病毒基因结构与功能分析[J].中国预防医学杂志,2009,10(1):72-75. 被引量:3
  • 2龚国忠,蒋永芳,何艳,赖力英,许允,苏先狮.丙型肝炎病毒非结构蛋白5A影响p53抑制肝癌细胞甲胎蛋白表达的分子机制[J].中华肝脏病杂志,2005,13(7):505-508. 被引量:2
  • 3段学章;张敏.丙型病毒性肝炎防治新进展[M]{H}北京:人民军医出版社,201057-65.
  • 4江家骥.丙型肝炎临床实践[M]{H}北京:人民卫生出版社,2010262-263.
  • 5Suppiah V,Moldovan M,Ahlenstiel G. IL-28B is as-sociated with response to chronic hepatitis C interferon-alpha and ribavirin therapy[J].{H}Nature genetics,2009.1100-1104.
  • 6Ge D,Fellay J,Thompson AJ. Genetic variation in IL-28B predigts hepatitis C treatment-induced viral clearance[J].{H}NATURE,2009.399-401.
  • 7Marrero JA, Scrag HB. Alpha-fetoprotein should be included in the hepatocellular careinoma surveillanee guidelines of the ameri- can association for the study of liver diseases [J]. Hepatology, 2011,53(3) : 1060.
  • 8吴孟超,孙燕,郑树森,等.原发性肝癌诊疗规范[M].北京:中华人民共和国卫生部,2011.
  • 9Kudo M. The 2008 oluda lecture management of hepatoeel-lular carcinoma:from surveill'anee to molecular targeted therapy[J]. J Gastroenterol Hepatol,2010,25(1) :439-452.
  • 10Shiraki K,Takase K, Tameda Y, et al. A Clinical study of lectin reactive alpha-fetoprotein as an early indict/tot of hepatoc elluar carcinoma in the follow up of cirrhotic patients[J]. Hepatology, 1995,22(1) :802-807.

引证文献3

二级引证文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部