新辅助化疗对口腔鳞癌环氧化酶2的影响及意义

Effects of neoadjuvant chemotherapy on COX-2 expression in oral squamous cell carcinoma and its significance

目的：探讨新辅助化疗对口腔鳞癌环氧化酶2（cycloxygenase 2,COX-2）、增殖细胞核抗原Ki-67表达和微血管密度（microvessel density,MVD）的影响及临床意义。方法：对从2004年1月-2007年6月间联合应用长春新碱和氨甲蝶呤行新辅助化疗的25例口腔鳞癌患者（辅助化疗组）与既往术前未作任何辅助治疗的28例口腔鳞癌患者（对照组）进行回顾性研究,应用免疫组织化学法检测两组肿瘤标本中的COX-2（光密度）、Ki-67（增殖指数）表达和微血管密度（MVD）。结果：辅助化疗组COX-2、Ki-67表达和MVD分别为（2.63±0.50）×10^5、（34.3±7.1）%和（25.6±4.2）条/HP,显著小于对照组的（3.47±0.67）×10^5、（44.6±9.6）%和（33.8±5.9）条/HP。COX-2与Ki-67和MVD均为正相关关系,相关系数分别为0.574（P〈0.0001）和0.462（P=0.0005）。结论：新辅助化疗可能通过抑制COX-2的表达及降低肿瘤细胞增殖活性,达到减少肿瘤新生血管生成的作用。

Objective： To explore the effects of neoadjuvant chemotherapy （ NAC ） on cycloxygenase 2 （ COX - 2 ） expression, Ki - 67 expression and microvessel density （MVD） in oral squamous cell carcinoma and its significance. Methods：25 patients with squamous carcinoma in oral cavity （NAC group ） were treated with vincristine and methotrexate chemotherapy before operation from January 2004 to June 2007. In control group,28 patients with the same carcinoma did not receive any NAC before operation in the past. Retrospective study was performed in both groups. The OD value of COX - 2, proliferation index of Ki - 67 and MVD were detected by i methods. Results ： COX - 2, Ki - 67 expressions and MVD in NAC group were （2.63 ± 0.50 ）× 10^5, （34.3 ± 7.1 ） % and （25.6± 4.2 ） micrangiums/HP respectively, which were all significantly lower than those in control group[ （3.47 ± 0.67 ） ×10^5 , （44.6 ±9.6 ） % and （ 33.8 ± 5.9 ） mierangiums/HP, respectively ]. Both COX - 2 and Ki - 67 expression had a positive correlation with MVD and the coefficient correlation was 0. 574 （ P 〈 0. 0001 ） and 0. 462 （ P = 0. 0005）, respectively. Conclusion ： NAC may reduce the tumor angiogenesis by inhibiting COX - 2 expression and decreasing the proliferation activity of tumor cells.