凋亡抑制因子Survivin在肺癌组织中的表达及临床意义

Expression and clinical significance of survivin,inhibitor of apoptosis proteins,in Lung cancer

目的探讨凋亡抑制蛋白Survivin在肺癌中的表达及其与临床病理参数的关系。方法应用免疫组织化学方法检测45例肺癌组织及肺部淋巴结中Survivin蛋白的表达。结果64.4%肺癌标本呈现为Survivin阳性表达,76.0%发生肿瘤细胞转移的淋巴结中有Survivin阳性细胞,而正常肺部组织及未发生肿瘤细胞转移的淋巴结中Sur-vivin表达率为0。Survivin阳性表达与肺癌患者年龄、组织学类型无明显相关,但与细胞分化程度、临床分期和淋巴结转移密切相关。分化程度为G1-G2级的肺癌标本中Survivin蛋白阳性表达率(50.0%)显著低于G3-G4级肺癌标本(78.3%,P<0.05);临床Ⅲ期肺癌患者Survivin蛋白阳性表达率(83.3%)明显高于临床Ⅰ、Ⅱ期患者(51.8%,P<0.05);淋巴结转移组的肺癌组织中Survivin蛋白阳性表达率(84.0%)显著高于而未转移组(40.0%,P<0.05)。结论Survivin在肺癌组织中高表达可能通过抑制细胞凋亡而在肺癌的发生发展中发挥一定作用。

Objective Investigate the expression of Survivin and its relationship with clinical pathological parameter.Methods Examine the expression of Survivin in 45 cases of lung cancer and lymph nodes in lungs with imunohistochemistry. Results 64.4% specimen appeared Survivin positive expression in lung cancer tissue, and 76.0% lymph nodes with tumor metastasis contained Survivin positive ceils. While in normal lung tissue and lymph nodes without tumor metastasis, the expression rate of Survivin is 0. The expression of Survivin had no relationship with age and histological type, but it was significantly associated with cell differentiation, clinical stage and lymph node metastasis. The positive expression rate of Survivin in grade G1-G2 （50.0%） was significantly lower than that in grade G3-G4 （78.3%,P 〈 0.05） ;The positive expression rate of Survivin in clinical stage m （83.3 % ） was significantly higher than that in stage Ⅰ and Ⅱ （51.8%, P 〈 0.05）;The positive expression rate of Survivin in lung cancer with lymph node metastasis（84.0 % ） was significantly higher than that in lung cancer without lymph node metastasis（40.0%, P 〈 0.05）. Conclusion The apoptosis inhibition caused by survivin protein may play an essential role in the origin and progression of lung cancer.