HPLC法对氟尼康中盐酸多西环素及氟苯尼考含量的测定

Content determination of Doxycycline hydrohloride and florfenicol in Funicon by HPLC

目的:建立了同时检测药物氟尼康中盐酸多西环素、氟苯尼考的高效液相色谱检测法。方法:采用Hypersil-ODS2(4.6mm×250mm,5μm)色谱柱,流动相为乙腈∶甲醇∶磷酸二氢铵(0.1mol/L)=10∶11∶29,pH=3.50,检测波长为254nm,流速为1ml/min。结果:实现了氟尼康中盐酸多西环素、氟苯尼考的较好分离;盐酸多西环素在6.25～200.00μg/ml范围内线性关系良好,r=0.9994,平均回收率为99.29%,RSD为0.51%(n=3);氟苯尼考在6.25～250.00μg/ml范围内线性关系良好,r=0.9999,平均回收率为98.87%,RSD为0.32%(n=3)。结论:该方法快速简便,灵敏度高,重现性好,可作为药物氟尼康质量标准的测定方法。

Objective：A method for determination of doxycycline hydrochloride and florfenicol in funicon was established. Methods：The liquid Chromatography was carried out by a Hypersil-ODS2 （4.6 mm×250 mm,5 μm）.The mobile phase was acetonitrile,methanol and 0.1 mol/L ammonium phosphate monobasic of pH 3.5（10：11：29） and the flow rate was 1 ml/ min. Funicon samples were measured with UV detector at 254 nm. Results：This experiment can get a better result of separation between doxycycline hydrochloride and florfenicol in funicon.The linear range of doxycycline hydrochloride was 6.25 μg/ml to 200.00μg/ml and florfenicol was 6.25 μg/ml to 250.00μg/ml （r=0.999 9）.The average recovery of doxyeycline hydroehloride was 99.29%,relative standard was 0.51%（n=3）. The average recovery of florfenicol was 98.87%,relative standard was 0.32%（n=3）.Condution：The method is simple,rapid and sensitive with good repeatability,can be used for content determination of funicon.