耐甲氧西林金黄色葡萄球菌相关耐药基因及耐消毒剂基因qacA的检测

Detection of the drug-resistant gene and disinfectant-resistant gene qacA in methicillin-resistant Staphylococcusaureus

目的对本院临床分离的多重耐药耐甲氧西林金黄色葡萄球菌(MRSA)耐消毒剂基因及抗生素耐药相关基因存在状况进行检测和分析,为避免多药耐药MRSA的流行提供依据。方法采用聚合酶链反应(PCR)技术检测45株MRSA中耐消毒剂基因(qacA)、β-内酰胺类耐药相关基因(mecA)、四环素耐药相关基因(tetM)、红霉素耐药相关基因(ermA/B/C)。结果45株MRSA中,qacA、mecAt、etMe、rm基因PCR扩增均阳性,阳性率为40%(18/45)、100%(45/45)、58%(26/45)、73%(33/45)。结论本院临床分离的多重耐药MRSA中qacA、mecAt、etM和erm基因携带率均很高,应引起医院感染监控部门高度重视,合理使用抗生素和消毒剂,避免多重耐药MRSA的进一步流行。

Objective It is to detect and analyze the existence condition of the disinfectant-resistant genes and antibiotic resistant genes in the multidrug-resistant and methicillin-resistant Staphylococcus aureus （MRSA） isolated from our hospital to supply evidence to avoid the spreading of MRSA. Methods The disinfectant-resistant gene of qacA, lactam-resistant gene of mecA, tetracyclines-resistant genes of tetM and erythromycin-resistant genes of emA/B/C in 45 strains of the MRSA were detected by poly-merase chain reaction（PCR）. Results The qacA, mecA, terM, erm genes were positive in the strains detected by PCR, the positive rates of them were 40% （18/45）, 100% （45/45）, 58% （26/45）, 73% （33/45） respectively. Conclusion The carrying rates of qacA, mecA, tetM, erm gene in the multidrug-resistant MRSA isolated from our hospital clinic were all high. Infection monitoring department of our hospital should pay high attention to this phenomenon. Reasonable use of antibiotic and disinfectant can avoid further speading of multidrug-resistant MRSA.