老年人肠道菌群检测及分析

Investigation on the intestinal microflora in the elderly

目的探讨老年人肠道菌群的特点。方法60岁以下健康成年人36例作为对照组,60岁及以上老年人68例作为老年组,分别取新鲜粪便,选择肠道菌群中7种具有代表性的细菌(包括4种厌氧菌、2种需氧菌和酵母样真菌)进行细菌培养和计数;计算出反映肠道定植抗力的指标B/E比值(双歧杆菌与肠杆菌数值比)。同时测定血清内毒素水平。结果与对照组相比,老年组肠道菌群中双歧杆菌数量减少[(10.17±0.81)lgCFU/gvs(9.08±1.13)lgCFU/g,P<0.01];肠杆菌[分别为[(8.19±0.68)lgCFU/gvs9.17±1.29)lgCFU/g,P<0.05]和肠球菌数量增加[(7.07±1.58)lgCFU/gvs(8.47±1.39)lgCFU/g,P<0.05];肠道定植抗力减低(1.24±0.18vs1.02±0.14,P<0.01)。结论老年人肠道菌群中双歧杆菌数量明显减少,肠杆菌、肠球菌数量明显增加;老年人肠道定植抗力下降。

Objective To investigate the characteristics of intestinal microflora of the elderly. Methods All 104 cases were assigned into two groups ： control group（ healthy adults, age 〈 60 years, n = 36） and elderly group （ the healthy elderly, age ≥ 60 years, n = 68 ）. Feces samples of all cases were cultured under both aerobic and anaerobic conditions for the 6 bacteria （4 kinds of anaerobes and 2 kinds of aerobes）and the yeasts, and the ratio of B/E was calculated. The serum endotoxin was also measured. Results Compared with control group, the number of fecal bifidobacteria and B/E ratio in elderly group significantly decreased [ fecal bifidobacteria： （ 10.17 ± 0.81 ） lgCFU/g vs （ 9.08±1.13 ） lgCFU/g, P 〈 0.01 ; B/E ratio： 1.24 ± 0.18 vs 1.02 ±0.14, P 〈 0.01 ] ; the numbers of Enterobacteria and Enterococcus significantly increased in geriatric group[ Enterobacteria ： （ 8.19 ± 0.68 ） lgCFU/g vs （ 9.17 ± 1.29 ） lgC- FU/g,P 〈0.05 ;Enterococcus： （7.07 ± 1.58）lgCFU/g vs（8.47 ±1.39）IgCFU/g,P 〈0.05]. Conclusion The elderly is characterized by the decreased number of fecal bifidobacteria, the increased number of Enterobacteria and Enterococcus and the decreased colo- nization resistance.