脱水敏感的黄皮种子在发育过程中的碳水化合物变化研究

Studies on the changes of carhydrates in the desiccation sensitive Wampee [Clausena lansium (Lour.) Skeels] seeds during development

脱水敏感的黄皮种子发育至生理成熟时淀粉积累达最大值，但其胚轴中淀粉含量远低于子叶且在种子成熟以后明显下降。花后65d子叶中可溶性糖含量最高而胚轴中可溶性糖随种子发育而一直上升。还原性葡萄糖和麦芽糖含量随种子发育而逐渐降低．非还原性果糖和蔗糖则逐渐增加。生理成熟期子叶中还含有较多的水苏糖和棉籽糖。外源ABA可提高成熟林子子叶中蔗糖的含量和未成熟种子的淀粉含量、但不能提高种子的耐脱水能力。因此我们认为黄皮种子的碳水化合物变化与其所在组织（子叶和胚轴）的不同代谢模式有关，而不是与种子的脱水敏感性有关。

In the investigation to Wampee seeds,the starch accumulated in either cotyledon or axis reached the maximum at physiological maturation of seeds and the starch content in axis was obviously declined after seed maturation and much lower than that in cotyledon. The content of soluble sugars in cotyledon was the highest at 65 days after pollination (DAP), but that in axls was going up all during seed development. In germination the starch was gradually degraded, but the soluble sugars changed dissimilarly in these two tissues. Analysed by HPLC the content of reduced g1ucose and maltose in seeds gradually declined with development, while the non-reduced fructose and sucrose showed a reverse change. In physiological matured cotyledon there also deposited relatively more stachyose and raffinose,Exogenous ABA could enhance the quantity of sucrose in cotyledon of rnatured seeds and the starch content in axis of prematured ones, but not improve the desiccation tolerance of seeds. So we speculated the carbohydrates in Wampee seeds might be associated with the different metabolic mode of their cotyledon and axis in development and gemination other than with their desiccation sensitivity.