针刺对促排卵治疗的多囊卵巢综合征大鼠子宫内膜容受性的影响(英文)

Acupuncture-induced improvement of endometrial receptivity of rats with polycystic ovary syndrome treated by Clomiphene for ovarian stimulation

目的:观察针刺对促排卵治疗的多囊卵巢综合征(PCOS)大鼠子宫内膜容受性的影响,探讨其作用机制。方法:皮下脱氢表雄酮(DHEA)的麻油溶液制作多囊卵巢综合征(PCOS)大鼠模型,对照组(Con)同期皮下注射麻油。PCOS大鼠随机分为模型组( M)、克罗米芬组(C)、克罗米芬+针刺组(C+A) ,其中M组不做任何处理,其他各组均于80日龄起进行干预5天。治疗结束后,各组部分大鼠断头处死,留取血清、子宫和子宫内膜标本。各组另部分大鼠与雄鼠交配,于妊娠第8天处死,观察大鼠胚泡着床情况。结果:与C组相比,C+A组大鼠血清E2水平较高,子宫内膜发育更好,子宫内膜容受性的标志分子蛋白及mRNA表达水平也更接近对照组(P<0·01) ,着床率和平均着床胚泡数也显著增加(P<0·01)。结论:针刺能显著改善克罗米芬促排卵治疗导致的子宫内膜容受性不良状态,促进胚泡着床,可能通过调节血清E2水平、子宫内膜容受性的标志分子蛋白及mRNA表达而实现。

Objective To observe acupuncture effects on endometrial receptivity of the rats with polycystic ovary syndrome （PCOS） treated by ovarian stimulation with Clomiphene to explore its mechanisms. Methods eGOS rat model was experimentally induced by subcutaneous injection of oil solution of dehydroepiandrosterone （DHEA） in immature （24-day-old） female rats for continuous 20 days. The same dose of oil was given for an equivalent length of time to animals in a control group （Group Con）. The rats with PCOS were randomly divided into a model group （group M）, a Clomiphene treatment group （group C）, a Clomiphene plus acupuncture group （group C＋ A）. The treatments were given for 5 days since 80 days after birth. Half of the rats were killed by decapitation after treatment finished, and the serum, uteri and endometria were obtained. The other half mated with male rats, and they were killed at the 8th day of pregnancy. We observed the implantation number and condition of pregnant uteri. Results Compared with group C, the animals in group C＋A had higher implantation rate and more average implanted embryos （P〈0. 01 ）, higher serum estradiol （E2） level （P〈0.01 ）, better development of endometrium, and increased mRNA and protein expressions of molecular markers of endometrial receptivity （P〈0.01）, which were closer to those in the control group. Conclusion Acupuncture could improve poor endometrial receptivity of PCOS rats with ovarian stimulation by citrated Clomiphene and promote implantation. The effects may be related with its up-regulation of serum E2 level, mRNA and protein expressions of molecular markers of endometrial receptivity.