香蕉炭疽病生防拮抗菌筛选及其作用机制

Screening and inhibitory mechanisms of antagonists for biological control of banana anthracnose fungus

用含炭疽病菌的ＰＤＡＹ平板上分离香蕉组织表面的拮抗菌株，经离体和活体测定对香蕉炭疽病的防效，筛选到９６００３、９６１２０、９６２０６、９５０４０、９６２０１、９５０１９、９５０４２及９６００４等８个有显著拮抗作用的菌株，其中９５０１９是酵母菌，其它为细菌．９６００３防效最佳，达６７％．供试菌株的拮抗作用机制可分为抑菌和竞争两类．

The antagonistic microbes were isolated from banana peel tissues on medium of PDAY mixed with spores of Colletotrichum musae. Eight isolates with good antagonistic effect were selected. Among them, one was yeast,the others were bacteria. The antagonistic mechanisms could be divided into fungusinhibition and competition. The inhibitory activity and antifungus metabolites of antagonistic isolates were analyzed. The results showed that some isolates inhibited the anthracnose fungus by secreting antifungus protein with thermostability. The isolate 96003 could decrease banana anthracnose disease by 67% constantly and steadily, showing good applicable prospect.