铜绿假单胞菌1型整合子与消毒剂抗性的研究

RELATION BETWEEN TYPE-1 INTEGRONS AND RESISTANCE TO DISINFECTANTS IN Pseudomonas aeruginosa

目的了解铜绿假单胞菌1型整合子携带情况及其与消毒剂抗性之间的关系。方法采用PCR技术对临床分离26株铜绿假单胞菌进行Ⅰ型整合子检测,用肉汤稀释法测定了上述菌株对消毒剂抗力。结果临床分离26株铜绿假单胞菌检出携带1型整合子16株,占61.5%。所有临床分离铜绿假单胞菌株对含碘消毒剂和苯扎氯铵两类消毒剂在常用使用浓度下均未产生抗性现象;但有38.5%的菌株对含氯消毒剂的M IC和MBC值增加。对含氯消毒剂M IC和MBC值增加的菌株中,有90%携带Ⅰ型整合子阳性。结论铜绿假单胞菌Ⅰ型整合子阳性携带率较高,其多数菌株对含氯消毒剂抗性增加。

Objective To investigate the carrying rate of type - 1 integron in Pseudomonas aeruginosa and its relation with resistance to common disfectants. Methods Type - 1 integrons in 26 stains ofP. aeruginosa isolated from burn units were detected by polymerase chain reaction （PCR） ; and the broth dilution method was used to determine the disinfectant - resistance of these isolates. Results Sixteen of the 26 isolates were identified to be type - 1 integron positive （61.5%）. All P. aeruginosa isolates had no resistance to the iodine - containing disinfectants and benzalkonium chloride; while the minimal inhibiting concentration （MIC） and minimal bactericidal concentration （MBC） of the chlorine - containing disinfectants for 38.5% isolated strains increased and 90% of which carried the type - 1 integrons. Conclusions The carrying rate of type - 1 integrons was high in P. aeruginosa isolates, the resistance of most of these strains to chlorine disinfectants was increased.