期刊文献+

抗阻训练对衰老小鼠心肌Sarcopenia中Akt/mTOR信号通路的影响 被引量:3

Effect of Resistance Training on Akt/mTOR Signaling Pathway of Aging Mice Cardiac Sarcopenia
下载PDF
导出
摘要 目的:探讨抗阻训练对衰老小鼠心肌sarcopenia中Akt/mTOR信号通路的影响。方法:雄性,3月龄SAMP8小鼠随机分为对照组(n=12)和抗阻训练组(n=12)。对照组常规喂养至6月龄,达到衰老后处死;抗阻训练组进行3个月的抗阻训练,建立衰老小鼠抗阻训练运动模型,使用实时荧光定量PCR技术,对小鼠心肌Akt/mTOR信号通路中Akt、mTOR、p70S6K和eIF-4E基因mRNA的表达进行测定。结果:衰老小鼠出现心肌纤维数目减少,心肌纤维空白区域面积增多;抗阻训练后,小鼠心脏重量和心系数与衰老对照组相比虽然显著增加(P<0.05)但没有产生病理性心肌肥大;心肌纤维空白区域面积小于对照组;抗阻训练组Akt、mTOR、p70S6K和eIF-4E基因mRNA的表达与衰老对照组相比显著上升(P<0.05)。结论:抗阻训练可以增强衰老小鼠心肌Akt/mTOR信号通路中各基因mRNA的表达,产生运动性心肌肥大,并对心肌产生保护性调节作用,改善衰老小鼠的心脏功能。 Objective: To study the effect of resistance training on cardiac sarcopenia. Methods: Male SAMP8 3-months old mice were randomly divided into control group (n= 12 ) and resistance training group (n= 12). The control group was executed at 6-months old. After inducing the animal model of resistance training, using the real-time PCR system to analyze the mRNA expression of Akt, mTOR, p70S6K and elF-4E in mouse heart. Result: The aging mice turned cardiac sarcopenia. After resistance training, the mouse heart weight and the ventricular mess index significantly increased (P〈0.05), without pathological cardiac hypertrophy, and did not turn cardiac sareopenia. The mRNA expressions of Akt, mTOR, p70S6K and elF-4E of resistance training group increased significantly than that of the aging control group (P〈0.05). Conclusion: The resistance training can elevate the cardiac P13-K/Akt signaling pathway expression, induce exercise cardiac hypertrophy, start the cardiac cell protection control action, and improve the cardiac function of aging mice.
出处 《天津体育学院学报》 CAS CSSCI 北大核心 2009年第4期334-336,365,共4页 Journal of Tianjin University of Sport
关键词 抗阻训练 心肌 SARCOPENIA resistance training cardiac sarcopenia
  • 相关文献

参考文献15

  • 1Jing Lin,Elizabeth F Lopez,Jin Yufang,et al.Age-related cardiac muscle sarcopenia:Combining experimental and mathematical modeling to identify mechanisms[J].Experimental Gerontology,2008,43(4):296-306.
  • 2Kotulska K,Larysz-Brysz M,Grajkowska W,et al.Cardiac rhabdomyomas in tuberous sclerosis complex show apoptosis regulation and mTOR pathway abnormalities[J].Pediatr Dev Pathol,2009,12(2):89-95.
  • 3Kemi O J,Ceci M,Wisloff U,et al.Activation or inactivation of cardiac Akt/mTOR signaling diverges physiological from pathological hypertrophy[J].Cell Physiol,2008,214(2):316-21.
  • 4Val é rioG,Barauna,Kaleizu T,et al.AT1 receptor participates in resistance training-induced cardiac hypertrophy in rats[J].Molecular and Cellular Cardiology,2007,42(6):S138.
  • 5Sukho Lee R P Farrar.Resistsace training induces muscle-specific changes in muscle mass and function in rat[J].Journal of Exercise Physiolog,2003,6:80-87.
  • 6Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta CT) method[J].Methods,2001,25(4):402-408.
  • 7Siu P M,Bryber R W,Martyn J K,et al.Apoptotic adaptations from exercise training in skeletal and cardiac muscles[J].FASEB,2004,8(10):1150-1152.
  • 8Solange M,Franzói-De-Moraes,S B Peres,et al.The Akt/PKB phosphorylation in cardiac muscle is modulated by an exercise training program[J].Diabetes Research and Clinical Practice,2000,50:375.
  • 9Li W,Tan D,Zhang Z,et al.Activation of Akt-mTOR-p70S6K pathway in angiogenesis in hepatocellular carcinoma[J].Oncal Rep,2008,20(4):713-9.
  • 10Dreyer H C,Glynn E L,Lujan H L,et al.Chronic paraplegia-induced muscle atrophy down regulates the mTOR/S6K1 signaling pathway[J].J Appl Physiol,2008,104(1):27-33.

同被引文献56

引证文献3

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部