两种小麦细胞质雄性不育体系mtDNA的RAPD分析

RAPD Analysis of mtDNA from Two Types ofCytoplasmic Male Sterility in Wheat

对Ｔ型和Ｖ型小麦细胞质雄性不育系ｍｔＤＮＡ的ＲＡＰＤ扩增产物分析结果表明，这两种不育系和其保持系间ｍｔＤＮＡ的ＲＡＰＤ扩增产物存在着显著差异，而两种保持系间的扩增产物则无明显不同，说明小麦细胞质雄性不育可能与ｍｔＤＮＡ的变异有关；同时，在两种不育体系的ＲＡＰＤ扩增产物之间，既有共性，又有差异，暗示这两种不育体系产生不育的机理可能不尽完全相同，而各具其特异性。

The differences of mtDNAs between Vtype and Ttype CMS systems in wheat were studied with RAPD technique.The results indicated that there were significant differences between the two male sterile lines and their maintainers in amplified DNA fragments of mtDNA,and there were no differences between the two maintainers in those of mtDNAs.It can be concluded that the cytoplasmic male sterility might have something to do with variation of mtDNA.In addition,both similarities as well as differences were expressed on the polymorphism products between the two CMS systems.It implied that the mechanisms of the CMS in wheat might be incompletely identical.The results confirmed the conclusion from the RFLP analysis before.