甘薯无病毒苗培育技术研究

TECHNIQUES FOR VIRUS-FREE SWEET POTATO CULTURE

1991～1996年,甘薯茎尖离体培养采用MS为基本培养基,添加6-苄基氨基嘌呤(6-BA)0.5mg/L+萘乙酸(NAA)0.2mg/L+腺素(Ad)5mg/L,培育出12个甘薯品种的脱毒苗,茎尖培养成苗率50.0％～95.6％,成苗期43～60d。切取的茎尖大小与脱毒效果呈显著的负相关(r=-0.9875),茎尖带1个叶原基时,SPFMV脱毒率为38.1％。培养基中添加适量的植物病毒钝化剂可使脱毒率提高到49.5％,但对成苗率有影响。采用Dot-ELISA血清学检测结合指示植物鉴定,能检出国内已知侵染甘薯的3种病毒,可得到茎尖组培脱毒苗。

12 sweet potato cultivars free from virus were obtained by meristem tip culture during 1991 - 1996. The MS agar medium used contained 0. 5 mg/L of 6-BA, 0. 2 mg/L NAA and 5 mg/L Ad. The plantlet ratio was 50. 5%-95. 6% from meristem tip. The form of plantlet took 43-60 d. The size of meristem tip cut was negatively related to the percentage of virus-free plantlet obtained (r =-0. 9875). The addition of 0. 015 mg/L of TS in medium exhibited the inhibitory effect on virus multiplication. It, however, had negative effect to the ratio of plantlet. SPFMV, SPLV and TMV can be detected using Dot-ELISA with SPFMV monoclonal antibody and TMV antiserum in combination with I. setosa and C. amaranticolor as identification hosts.