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环介导等温扩增芯片核酸最佳扩增条件研究 被引量：1

Optimal DNA amplification of lab-on-a-chip for loop-mediated isothermal amplification
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摘要 研制了一款环介导等温扩增芯片,开发了相应的检测控制系统,并在此芯片上进行了金葡菌环介导等温扩增反应最佳扩增条件的研究.实现了在聚碳酸酯芯片上进行环介导等温扩增实验,与传统管式聚合酶链式反应相比,它需要的样品和试剂量更少.通过在不同温度下进行实验,获得了在芯片微体系条件下,进行环介导等温扩增反应检测金葡菌的最佳温度条件为61℃,提高了环介导等温扩增芯片的检测速度,缩短了核酸分析时间. A section of loop-mediated isothermal amplification （LAMP） chip was introduced and its detecting platform was developed. Optimal DNA amplification of the detecting and controlling platform on the LAMP chip was studied. Using this chip and detecting platform, the lung infection bacterial staphyloccocus aureus rosebush was tested. Compared with conventional tubular polymerase chain reaction, more accurate results with smaller amount of samples and reagents were obtained by this chipbased LAMP analysis. The optimal experimental condition of isothermal DNA amplification of lab-on- a-chip （LOC） is obtained by the experiment, under which DNA detection of LOC is improved effectively and the time of DNA analysis is shortened.
作者 罗磊 阳小勇 于军
机构地区 华中科技大学电子科学与技术系 生物芯片北京国家工程研究中心
出处 《华中科技大学学报（自然科学版）》 EI CAS CSCD 北大核心 2009年第7期42-45,共4页 Journal of Huazhong University of Science and Technology(Natural Science Edition)
基金 国家高技术研究发展计划资助项目(2006AA020701 2006AA020803) 国家重点基础研究发展计划资助项目(2006CB705700) 浙江省科技攻关重点项目(2006C21G3210005) 清华-裕元医学科学研究基金资助项目(40000510B)
关键词 芯片实验室 生物芯片 检测技术与仪器 环介导等温扩增 最佳实验条件 lab-on-a-chip （LOC） biochip detecting technology and apparatus loop-mediated isothermal amplification optimal experimental condition
分类号 TP212 [自动化与计算机技术—检测技术与自动化装置] TP27 [自动化与计算机技术—检测技术与自动化装置]
  • 相关文献

参考文献10

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同被引文献100

  • 1熊槐,吴凡,冯雪梅,黄昱阳,杜正平,周毅,田文武,曹以诚.改良LAMP法检测转基因作物[J].食品安全质量检测学报,2012,3(3):177-181. 被引量：5
  • 2王清华,徐君怡,曹冬梅,刘宇,曹际娟.转基因玉米BT11品系环介导等温扩增(LAMP)检测方法的建立[J].食品安全质量检测学报,2013,4(3):868-872. 被引量：5
  • 3邢宇俊,吴季荣,徐剑宏,史建荣.环介导等温扩增技术检测转基因组分的研究进展[J].食品安全质量检测学报,2014,5(3):853-860. 被引量：3
  • 4申建维,王旭,范春明,孙秀琴,杜万英,万玉梅,彭铁汉,袁玮.多重分子信标环介导等温扩增快速检测耐甲氧西林金黄色葡萄球菌[J].中华医院感染学杂志,2006,16(7):729-733. 被引量：20
  • 5Notomi T, Okayama H, Masubuchi H, et al. loop-mediated isothermal amplification of DNA[J]. Nucl Acids Res, 2000, 28 (12): e63.
  • 6Tomita N, Moil Y, Kanda H, et al. Loop-mediated isothermal amplification(LAMP)of gene sequences and simple visual detection of products[J]. Nat Protoc, 2008, 3(5): 877-882.
  • 7Mori Y, Nagamine K, Tomita N. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation[J]. Biochem Bioph Res Co, 2001, 289 (1): 150-154.
  • 8Fukuta S, Kato S, Yoshida K, et al. Detection of tomato yellow leaf curl virus by loop-mediated isothermal amplification reaction[J]. J Virol Methods, 2003, 112(1-2): 35-40.
  • 9Thai H T C, Le M Q, Vuong C D, et al. Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus[J]. J Clin Microbiol, 2004, 42 (5): 1 956-1 961.
  • 10Mori Y, Kitao M, Tomita N, et al. Real-time turbidimetry of LAMP reaction for quantifying template DNA[J]. J Biochem Biophys Methods, 2004, 59(2): 145-157.

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华中科技大学学报（自然科学版）
2009年 第7期

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