精氨酸与N^G-硝基-精氨酸甲酯对大鼠正畸牙移动时诱导型一氧化氮合酶的影响

The influence of L-arginine and N^G-nitro-L-arginine methyl ester synthase expression during orthodontic tooth movement in rats

目的研究精氨酸(L-arg)与NG-硝基-精氨酸甲酯(L-NAME)对大鼠牙周膜压力侧诱导型一氧化氮合酶(iNOS)的影响。方法将60只雄性S-D大鼠随机分为3组并建立正畸牙移动模型,分别于局部骨膜下注射精氨酸(L-arg组)、生理盐水(生理盐水组)和NG-硝基-精氨酸甲酯(L-NAME组),每2d1次。定期处死动物,取材并制作组织切片,进行免疫组化染色和图像分析。结果各实验组iNOS的表达-时间曲线基本相似,均在加力后7d达到高峰,牙周膜压力侧iNOS的表达在同一时间点不同实验组之间比较差异无统计学意义。结论L-arg和L-NAME对大鼠正畸牙移动牙周膜内iNOS的表达并无影响,两者可能通过其他途径影响正畸牙牙周膜的改建和正畸牙的移动。

Objective To study the influence of L-arginine （L-arg） and N^G-nitro-L-arginine methyl ester （L- NAME） on inducible nitric oxide synthase （iNOS） expression in compressed periodontium during orthodontic tooth movement in rats. Methods 60 male S-D rats were randomly divided into 3 groups and rat models with orthodontic appliances were established. Three groups received respectively subperiosteal injections of L-arg （L-arg group）, normal saline（NaCl group） and L-NAME （L-NAME group） at 2-day intervals. Rats were sacrificed at given time points. The maxilla tissue samples were collected and sliced up for immunohistochemistry and image analyses. Results All groups showed a similar iNOS expression after orthodontic force load and iNOS expression reached the maximum at day 7. No statistically significant differenee was found in iNOS expression between different study groups at the same time point. Conclusion Both L-arg and L-NAME have no effect on the expression of iNOS in periodontal ligament during orthodontic tooth movement in rats. They may affect orthodontic periodontium remodeling and orthodontic tooth movement through some other ways.