海地瓜的酶水解液的抗氧化活性研究

Study on the antioxidation activity of Acaudina leucoprocta enzymatic hydrolysate

目的:通过观察海地瓜体壁的亚显微结构,对它的质地做出合理的解释,选择合理的加工技术;探讨海地瓜的水解产物的体内抗氧化作用。方法:用电镜扫描法观察海地瓜体壁结构;通过正交实验确定复合蛋白酶酶解海地瓜的最适条件。制得海地瓜水解液,灌胃大鼠。56d后,测定大鼠血清和肝脏组织匀浆液中SOD,GSH-Px活力。结果与结论:电镜扫描分析发现海地瓜的肌纤维丝较粗壮,排列凌乱,纤维丝排列紧密。海地瓜肌肉组织的这种排列方式可能是它的肌肉坚韧,难于咀嚼的原因。正交实验确定海地瓜的最佳酶解条件为:2%复合蛋白酶,10%底物,酶解温度55℃,酶解时间70min。水解液稀释一倍,灌胃大鼠。发现灌胃海地瓜水解液的大鼠血液中SOD和GSH-Px的活力和肝脏组织中SOD活力比较空白组具有显著提高,说明海地瓜水解液具有良好的抗氧化活性。

Objective： To make a reasonable explaination of body walltexture of Acaudina by observing the ultrastructure. To investigate the antioxidation activity of Acaudina leucoprocta hydrolysate. Method： Acaudina leucoprocta texture was observed by scanning electron microscope; Determine the optimum condition of hydrolysis with the compound protease through the orthogonal experiment. The activity of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in blood serum and liver tissue in mice were analyzed after 56 days of hydrolysate feeding. Result and Conclution： The scanning electron microscope analyses of Acaudina leucoprocta showed that its muscle fiber were thick, in a mess and the fibrils arranged densely. Orthogonal experiment indicated that the rational conditions of enzymatic hydrolysis were： complex protease was 2%, substrate concentration was 10%, the optimal temperature was 55 ℃ and enzymolysis time was 70 min.Then the enzymolysis liquid was diluted with water in the ratio of 1：1. Feeding the mice by gastric perfusion. The results was that the activities of SOD, GSH-Px were enhanced, which showed that this hydrolysate had remarkable antioxidation activity.