牛卵巢腔前卵泡的机械分离试验

Mechanical Isolation of Preantral Follicles in Bovines

机械方法处理胎牛、犊牛和成年牛卵巢可以获得大量的腔前卵泡。胎牛卵巢用眼科手术剪刀剪碎（Ｍ１）或用组织切碎机切碎（Ｍ２），犊牛和成年牛卵巢用组织切碎机切碎，经悬浮吹打和过滤，平均每只卵巢可获得腔前卵泡胎牛为３２８２（Ｍ１）和５６８８（Ｍ２），犊牛为２１００，成年牛为３５２．在所分离的胎牛和犊牛卵巢卵泡中原始卵泡和初级卵泡占绝大部分，而成年牛卵巢卵泡主要是初级卵泡和次级卵泡。不论是胎牛、犊牛或成年牛。

A large number of preantral follicles had been obtained by mechanical procedure from bovine fetuses,calves and cows.The ovaries of fetuses were cut into small pieces (≤1mm) with both an optical scissors (M1) and a tissue chopper (M2),and ovaries of calves and cows were cut with a tissue chopper.Then the suspensions of ovarian fragments were suspended and filtered.The mean numbers of preantral follicles isolated from each ovary of fetuses,calves and cows were 3282 (M1) and 5688 (M2),2100 and 352 respectively.The vast majority of the ovarian follicles isolated from fetus and calf ovaries consisted of primordial follicles and primary follicles,and mainly primary and secondary follicles from cow ovaries,most of which had good quality.