灵芝孢子粉对亚硝酸钠致果蝇畸变效应的抑制作用研究

Antagonistic Effects of Ganoderma Spore on Mutation Induced by Sodium Nitrite in Drosophila melanogaster

目的研究灵芝孢子粉对亚硝酸钠诱发的黑腹果蝇畸变效应的抑制作用。方法将野生型雄性黑腹果蝇饥饿处理24h,接着转移到0、0.001、0.01、0.05和0.1g/L的亚硝酸钠培养基里培养24h,再加入雌果蝇培养7d。以0、0.2%、1%、2%的去壁和未去壁灵芝孢子粉培养经0、0.01、0.1g/L亚硝酸钠染毒24h后的果蝇7d,以基本培养基培养的果蝇为阴性对照。统计子代果蝇数和畸变数,并计算畸变率。结果畸变的果蝇主要以残翅居多,还有一些是白体,极个别的眼睛发生畸变。随着亚硝酸钠染毒浓度的增加,子代果蝇数呈下降趋势,畸变率呈上升趋势。随着未去壁灵芝孢子粉染毒浓度的增加,子代果蝇数呈下降趋势。与阴性对照组相比,0.1、0.01g/L亚硝酸钠+0.2%的未去壁灵芝孢子粉组子代果蝇畸变率均较高,差异有统计学意义(P<0.05或P<0.01)。与阴性对照组相比,0.01g/L亚硝酸钠+1%,2%的去壁灵芝孢子粉组和0.1g/L亚硝酸钠+0.2%的去壁灵芝孢子粉组子代果蝇数均较多,差异有统计学意义(P<0.05或P<0.01)。随着去壁灵芝孢子粉染毒浓度的增加,子代果蝇畸变率呈下降趋势。结论灵芝孢子粉可抑制亚硝酸钠诱发的果蝇畸变效应。

Objective To study the antagonistic effects of Ganoderma spore on mutation induced by NaNO2 in Drosophila melanogaster. Methods After 24 hours of starvation, male Drosophila rnelanogaster were transferred to the culture bottles with medium with NaNO2 of 0,0.001,0.01,0.05 and 0.1 g/L for 24 hours and then cultured with female Drosophila melanogaster for 7 days. Male Drosophila melanogaster were treated with NaNO2 at the doses of 0,0.01,0.001 g/L for 24 hours and then transferred to the medium with Ganoderma spores at the dose of 0.2%, 1% ,2% ,cultured with female Drosophila melanogaster for 7 days. The distilled water was used as the negative control. The amount of F1 generation and the mutation of the F1 generation were calculated. Results The mutation Drosophila melanogaster were mainly vestigial, some had malformation with white body and strange eyes. Compared with the negative control group, the count of offspring decreased, the rate of mutation proved positively correlated to the concentration of NaNO2. High dose of sporoderm-unbreken spores could cause a decrease of number of offspring. Compared with the negative control group,the mutation in the group of 0.1,0.01 g/L NaNO2 with 0.2% sporooderm-unbroken spores was much higher （P〈0.01 or P〈0.05）. Compared with the negative group,number of offspring in the group of 0.01 g/L NaNO2 with 1% ,2% sporoderm-broken and the group of 0.1 g/L NaNO2 with 0.2% sporoderm-broken increased（P〈0.01 or P〈0.05）. The rate of mutation proved negatively correlated to the concentration of sporoderm-broken spores. Conclusion Ganoderma spore has a significant antagonistic effects on mutation induced by NaNO2 in Drosophila melanogaster.