黑苞橐吾挥发油成分的GC-MS分析

GC-MS Analysis of the Chemical Components of Essential Oils in Ligularia melanocephala

目的:通过研究黑苞橐吾挥发油的化学成分,为黑苞橐吾的开发利用提供理论依据.方法:采用水蒸气蒸馏法提取黑苞橐吾挥发油,用GC毛细管柱进行分析,归一化法测其相对含量,并用气相色谱-质谱法对化学成分进行鉴定.结果:共鉴定出65个化合物,鉴定出的化合物含量占总挥发油的76.75%.黑苞橐吾挥发油主要成分为:[E,E,E]-3,7,11,15-四甲基-1,3,6,10,14-十六碳五烯(相对含量为5.19%);2-[1-环戊-1-烯炔-1-甲基乙基]环戊酮(4.38%);[1S-[1α,3aβ,4α,8aβ]]-4,8,8-三甲基-9-亚甲基-十氢-1,4-甲醇薁(4.10%);D-柠檬烯(4.02%);[2R-[2,α4aα,8aβ]]-十氢-α,α,4a-三甲基-8-亚甲基-2-甲醇基萘(3.93%);正-三十四烷(3.49%);3-[1,1-二甲基丙-2-烯-7-羟基香豆素(3.03%);石竹烯氧化物(2.72%);5,β7-βH,10-α11-桉叶烯-1α-醇(2.66%);4[10]-崖柏烯(2.56%).

In order to provide the useful information for the reasonable utilization of Ligularia melanoceph- ala, the essential oils of the herb were extracted with steam distillation and separated with GC capillary columns. The components were quantitatively determined with the normalization method and identified with GC-MS. A total of 62 components were identified, accounting for 75.66%of the total contents of es- sential oils. The main components of the essential oils were [E, E, El-3,7,11,15-tetramethylhexadeca-1, 3,6, 10, 14-pentaene, 2-[- 1-Cyclopent-l-enyl-l-methylethyl] cyclopentanone, 1,4-Methanoazulene, deca- hydro-4,8, 8-trimethyl-9-methylene, [1S-[1. alpha. , 3a. beta. , 4. alpha. , 8a. beta. ]]）, D-Limonene, 2- Naphthalenemethanol, decahydro-, alpha. ,. alpha. , 4a-trimethyl-8-methylene-, [2R-I-2. alpha. , 4a. alpha. , 8a. beta. ]], n-Tetratriacontane, 7-Hydroxy-3-[-1,1-dimethylprop-2-enyl] coumarin, Caryophyllene oxide, 5. beta. , 7. beta. H, 10. alpha.-Eudesm- ll-en-1, alpha.-ol and 4 [-10 ]-Thujene, accounting 5.19%, 4.38%, 4. 10%, 4.02%, 3.93%, 3.49%, 3.03%, 2.72%, 2.66% and 2.56%of the total.