IGF-Ⅰ和hCG对山羊睾丸间质细胞分泌睾酮的影响

Effects of IGFⅠ and hCG on TestosteroneSecretion in Cultured Goat Leydig Cells

采用体外培养山羊睾丸间质细胞的方法，应用ＩＧＦ－Ⅰ和ｈＣＧ进行不同处理，利用放射免疫分析法（ＲＩＡ）测定睾酮含量。结果表明：①３０～４０日龄山羊睾丸间质细胞适宜作为体外培养的材料；②山羊睾丸间质细胞的敏感性随ｈＣＧ的重复刺激而下降；③ＩＧＦ－Ⅰ能够促进睾酮的分泌（Ｐ＜０．０５～Ｐ＜０．０１）；④ＩＧＦ－Ⅰ和ｈＣＧ对睾酮的分泌具有协同促进作用（Ｐ＜０．０５～Ｐ＜０．０１）。

With the method of in vitro goat leydig cell culture and different treatments of IGFⅠ and hCG,the contents of testosterone have been determined by RIA.The results showed that: (1) Leydig cells from 30～40dayold goats are appropriate materials for in vitro study. (2) The goat Leydig cell was sensitive to hCG stimulation on testosterone production,but the sensitivity declined with the repeated hCG treatments.(3) IGFⅠ enhanced both basal and hCGinduced testosterone secretion (P<0.05～P<0.01).(4) hCG and IGFⅠ had accelerative function to testosterone secretion (P<0.05～P<0,01).