表征高分子形态、表面和界面的新方法-激光扫描共聚焦荧光显微镜法(LSCFM)

A New Method for Characterizing the Surface, Interface and Morphology of Polymer-Laser Scanning Confocal Fluorescence Microscopy(LSCFM)

从原理和应用两方面介绍了激光扫描共聚焦荧光显微镜法(LSCFM)。LSCFM是一种亚微米级荧光成象法,有四个因素影响其灵敏度:聚焦、物镜、探测器和光源。与传统的表征高分子形态、表面和界面的方法相比,LSCFM具有精度较高、制样简单且不损伤样品、快速反应、可三维成象的特点;大量的LSCFM应用研究表明,LSCFM是一种国外正在发展的,在药物控释、高分子材料形态、表面和界面表征中逐渐得到广泛应用的新方法。

The principle and the applications of laser scanning confocal fluorescence microscopy （LSCFM） are introduced. LSCFM is a fluorescence microscopy with sub-micrometer precision. There are four factors which affect the sensitivity of detection： focusing, object lens, detector and light source. Comparing with the conventional methods for characterizing the morphology, the sudace and the interface of polymer, LSCFM possesses the advantages of high precision, easily making samples without damage, instant reaction and 3-D imaging. A large amount of investigations on the application of LSCFM show that LSCFM is a novel method which is still developing abroad and widely applied in the area of controlling drug release, characterization of the morphology, surface and interface of polymer material.