检测禽霍乱抗体间接ELISA法的建立

Establishment of Indirect ELISA forDetecting Fowl Cholera Antibody

以方阵滴定法选择出最适ＥＬＩＳＡ反应条件，用系列稀释法测定２１份血清样品的禽霍乱抗体ＥＬＩＳＡ效价（ＥＴ），并与相应血清样品１∶２００倍稀释的Ｐ／Ｎ值配对，作线性回归分析，得到回归方程ｙ＝２１９．６７３ａ－１８３．５７０（ｒ＝０．９４４）和标准曲线，从而建立起快速定量测定禽霍乱抗体的间接ＥＬＩＳＡ方法——阳阴比值ＥＬＩＳＡ效价法（Ｐ／ＮＩ－ＥＴ法）。利用该法，血清样品只需作１∶２００倍稀释，测定其Ｐ／Ｎ值，通过回归方程（或标准曲线）即可求得ＥＴ．

Using the method of chessboard titration to choose the proper reaction condition,after a series of dilution,21 samples of chicken serum were detected for their fowl cholera (FC) antibody ELISA titer (ET).Paired with ET and the P/N value of 1: 200 dilution and after linear regression analysis,a linear equation y=219.673x-183.570 (r=0.944) and a standard diagram of curves were got.Thus an indirect ELISA which could rapidly and quatitatively detect FC antibodyP/NET method was established,with which the ET could be canculated out with regression equation only if the serum samples were diluted at 1∶200 and P/N value determined.