塞来昔布对胶质瘤U251细胞株放射敏感性的影响

Effect of celecoxib on the radiosensitivity of U251 glioma cell lines

目的:探讨环氧化酶-2抑制剂塞来昔布(celecoxib)对胶质瘤放射治疗的增敏作用及可能的作用机制。方法:体外培养胶质瘤U251细胞株并将其分为5组,分别加入不同浓度的塞来昔布,并采用60Go照射,噻唑蓝检测细胞的抑制率,免疫组织化学法测细胞表皮生长因子受体(EGFR)的表达,RT-PCR检测毛细血管扩张性共济失调症突变蛋白(ATM)的表达变化。结果:随着塞来昔布浓度的增加,细胞生长的抑制率明显上升,肿瘤细胞中ATM和EGFR蛋白的表达明显减弱。结论:塞来昔布能够通过降低ATM和EGFR蛋白的表达,增强胶质瘤的放疗敏感性,对胶质瘤的治疗具有重要意义。

Objective To investigate the effect of cyclooxygenase-2 inhibitor celecoxib on the radiosensitivity of U251 glioma cell lines. Methods U251 glioma cells were divided into 5 groups administered by different concentrations of celecoxib, then were exposed to cobalt 60 radiation. The inhibitive rates of U251 glioma ceils were measured by MTT assay, expressions of epidermal growth factor receptor （EGFR） were evaluated by immunohistochemistry method, and ataxia telangiectasia mutated （ATM） was detected by RT-PCR. Results The inhibitive rates were increased and the expressions of ATM and EGFR were decreased with the increase of concentrations of celecoxib. Conclusion Celecoxib can reduce the expressions of ATM and EGFR, then increase the radiosensitivity of glioma cells.