CD4^+CD25^(high) Tr细胞在非小细胞肺癌中的表达及意义

Expression and significance of CD4^+CD25^(high) regulatory T cells in patients with non-small-cell lung cancer

目的:比较非小细胞肺癌(NSCLC)患者外周血和肿瘤浸润淋巴细胞(TIL)中CD4+CD25high Tr细胞比例的变化与肺癌分期的关系,探讨患者局部肿瘤微环境和全身免疫状态与CD4+CD25+Tr细胞的关系。方法:分离NSCLC患者和健康人外周血单个核细胞(PBMC),取NSCLC患者新鲜肺癌组织抽提TIL。用流式细胞仪检测CD4+T细胞和CD4+CD25high Tr细胞。结果:NSCLC患者PBMC中CD4+CD25high Tr/CD4+T细胞比例较健康人显著升高(P<0.01)。Ⅱ～ⅢA期NSCLC患者PBMC和TIL中CD4+CD25high Tr/CD4+T细胞比例较Ⅰ期患者显著升高。结论:CD4+CD25high Tr细胞可能参与肿瘤产生的宿主免疫抑制反应及其发展。

Objective To investigate the proportion of CD4^＋CD25^high regulatory T （CD4^＋CD25^high Tr） cells in peripheral blood mononuclear cells （PBMC） and tumor-infiltrating lymphocytes （TIL） in patients with non-small-cell lung cancer （NSCLC）, and to evaluate the relationships between tumor microenvironment or general immunosuppressive status and CD4^＋CD25^high Tr cells. Methods PBMC were isolated from 20 patients with NSCLC and 20 healthy controls, and TIL were isolated from 20 patients with NSCLC. CD4^＋ T cells and CD4^＋CD25^high Tr cells were assessed by flow cytometry. Results The proportion of CD4^CD25^high Tr/CD4^＋ T in PBMC was significantly higher in patients with NSCLC than that in healthy controls（P 〈 0.01 ）. The percentages of CD4^＋CD25^high Tr/CD4^＋＋ T in PBMC and TIL were significantly higher in patients with NSCLC Ⅱ-Ⅲ A stage than those in patients with NSCLC Ⅰ stage （P 〈 0.01 ）. Conclusion CD4^＋CD25^high Tr cells may be involved in the immunosuppressive response induced by tumor, and may play an important role in the development of tumor.