雏鸡实验感染乙型脑炎和登革病毒的研究

Experimental Infection of Chicken withJapanese Encephalitis and Dengue Viruses

用乙型脑炎（ＪＥ）和登革４型（ＤＥＮ４）病毒人工感染雏鸡，结果表明，雏鸡能产生１～４天的ＪＥ病毒血症和１～５天的ＤＥＮ４病毒血症；ＪＥ血凝抑制（ＨＩ）抗体和中和（ＮＴ）抗体于第６～８天产生，ＤＥＮ４的ＨＩ和ＮＴ抗体于第８～１０天产生，两种病毒的特异性抗体均于１６～２０天达到高峰。感染蚊虫叮咬，能将ＪＥ和ＤＥＮ４病毒传给雏鸡。实验证明，雏鸡对这两种病毒敏感。认为雏鸡在ＪＥ和ＤＥＮ病毒的保存和扩散中起一定作用。

Postinoculated with Japanese encephalitis(JE,Nakayama and E18 strains)and dengue type 4(DEN4,H241 and Ban18 strains),chickens showed JE viremia 1～4 days and DEN4 viremia 1～5 days,but Hemagglutination inhibition(HI) and Neutralization(NT) antibodies against JE virus appeared on the 6th～8th day.HI and NT antibodies against DEN4 virus occured on the 8th～10th day after infection.Specific antibodies reached peak value on the 16th～20th day.Mosquitoes for 10th day after infection were capable of transmitting JE and DEN4 viruses by bite to chickens.Experimental studies showed that chicken is a susceptible animal to JE and DEN viruses,and has some role in the maintenance and amplification of JE and DEN viruses in nature.