膀胱移行细胞癌VEGF的表达及其意义

Expression of VEGF in transitional cell carcinoma of bladder and its significance

目的:探讨VEGF在膀胱移行细胞癌(BTCC)中的表达及其与BTCC生物学行为的关系。方法用流式细胞技术(FCM)对40例BTCC和20例正常膀胱黏膜细胞组织标本进行VEGF阳性细胞检测。结果BTCC组织中VEGF阳性细胞比率为(34.50±15.57)%,在正常膀胱黏膜组织中的VEGF阳性细胞比率为(13.30±6.23)%,两者比较差异有统计学意义(P<0.01);临床分期Tis^T1为(27.07±10.76)%,T2~T4为(48.29±13.77)%,病理分级Ⅰ级为(22.4±38.15)%,Ⅱ级为(35.01±9.95)%,Ⅲ级为(53.09±12.39)%;随着分期分级的增加,VEGF的表达率也逐步增加,差异有统计学意义(P<0.01)。结论VEGF定量分析可作为BTCC的生物学行为评估方法之一。

Objective To investigate the quantitative expression of VEGF in transitional cell carcinoma of bladder and to evaluate the relationship between VEGF and the biological behavior of transitional cell carcinoma, of bladder （BTCC）. Methods Samples of tumor and normal mucosa were taken from 40 patients and 20 cases of normal bladder tissue respectively. The percentage of VEGF positive cells in these samples were examined by flow cytometry. Results The percentage of VEGF positive cells was （34.50 ± 15.57） 96 in tumor tissue and （13.30 ± 6.23） % in normal tissue （P〈0.01）. The positive cells were noted in （27.7 ± 10.76） % of Tis--T1 and （48.29 ± 13.77） % of T2--T4, and （22.43±8.15）% orgy, （35.01±9.95）% of G2, and （53.09±12.39）% of G3 tumor tissue. With the progression of clinical stage and grade, the positive rate of VEGF expression increased （ P 〈 0.01 ）. Conclusions Quantitative examination of VEGF expression can be one method to evaluate the biological behavior of BTCC.