水貂Mustla Vison微量血DNA的提取与纯化

Extraction and Purification of DNA From Microblood of mink

作者通过采取活体水貂少量血液 ( 5 0 μL) ,利用蛋白酶K消化 ,酚、氯仿法抽取DNA ,结果测量获得的数据和曲线表明 :用 5 0～ 5 0 0 μL冻存血可以获得具有一定纯度和数量的DNA 。

Taking microblood from live mink body,which digested by proteinase K and extraction and purification of DNA by phenol chloroform method.Gaind data and curve showed that using 50～500 μL blood can receive fixed quantity DNA and have fixed purity,which can satisfy the needs of RAPD analysis.