纤维结合蛋白和巨噬细胞移动抑制因子在大鼠重症急性胰腺炎相关肺损伤中的作用

Effects of MIF and FN on rats with severe acute pancreatitis associated lung injury

目的探讨纤维结合蛋白(FN)和巨噬细胞移动抑制因子(MIF)在大鼠重症急性胰腺炎相关肺损伤发病机制中的作用。方法30只雌性SD大鼠随机分为正常对照组、胰腺炎模型2、4、8和12h组。采用5%牛磺胆酸钠逆行胰胆管注射的方法,复制大鼠重症急性胰腺炎相关肺损伤模型。分别对胰腺、肺损伤程度进行病理评分,测定肺组织湿/干质量比、胰腺组织湿重、血清淀粉酶;用ELISA法检测血浆FN和血清MIF含量,用RT-PCR法检测肺组织FNmRNA和MIFmRNA表达水平。结果造模后各组肺、胰腺损伤评分,肺组织湿/干质量比,胰腺组织湿重及血清淀粉酶均逐渐升高。造模后各组血浆FN及肺组织FNmRNA较正常组降低,而血清MIF及肺组织MIFmRNA较正常组升高。结论大鼠重症急性胰腺炎相关肺损伤发病过程中,血清及肺组织MIF表达明显增加,血浆及肺组织FN表达明显降低,提示MIF和FN与重症急性胰腺炎相关肺损伤的发生可能有关。

[Objective] To study effects of FN and MIF on rats with severe acute pancreatitis associated lung injury. [Methods] Thirty female Sprague-Dawley （SD） rats were divided into normal group, model groups （2 hours group, 4 hours group,8 hours group and 12 hours group）. 1 mL/kg body weight of 5% sodium taurocholate was retrograde injected into the biliopancreatic duct of the rats to induce SAP. Microscopic scores of pancreas and lung injury were evaluated. Blood were obtained for detecting plasma amylase. Pancreatic wet weight, lung wet/dry weight ratio were detected, plasma FN and serum MIF were detected by ELISA, FNmRNA and MIFmRNA expression in lung were detected by RT-PCR. [Results] Microscopic scores of pancreas and lung injury, blood amylase, Pancreatic wet weight, lung wet/dry weight ratio of rats in all model groups gradually increased. FN in plasma and FNmRNA expression in lung were less than normal group, but MIF in serum and MIFmRNA expression in lung was more than norreal group. [Conclusion] In the couse of rats SAP with lung injury, MIF was more expressed in serum and lung, FN was less expressed in plasma and lung, which suggests MIF and FN probably have some relations with the severe acute pancreatitis associated lung injury.