高迁移率族蛋白A1在子宫内膜癌中的表达及意义

Expression and significance of high mobility group protein A1 in endometrial carcinoma

目的探讨高迁移率族蛋白A1(high mobility group protein A1,HMGA1)在子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及子宫内膜癌组织、正常子宫内膜组织中的表达及意义。方法采用半定量RT-PCR和WesternBlot检测子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及21例子宫内膜癌组织(研究组)、18例正常子宫内膜组织(对照组)中HMGA1-mRNA与HMGA1蛋白的表达水平。结果HM-GA1-mRNA与HMGA1蛋白在子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及两组子宫内膜组织标本中均有不同程度的表达,研究组中HMGA1-mRNA与HMGA1蛋白的平均表达水平显著高于对照组(P<0.05),并与手术-病理分期有关(P<0.05),与子宫内膜癌组织的分化程度无关(P>0.05)。结论HMGA1的过度表达可能参与了子宫内膜癌的发生和发展,在子宫内膜癌细胞的增殖与侵袭中可能发挥重要作用。

[Objective] To investigate the expression and significance of high mobility group protein A1 （HMGA1） in endometrial carcinoma cell lines AN3CA, ECC-1, Ishikawa and tissue specimens of carcinomatous endometrium and normal endometrium. [Methods] The expression of HMGAI-mRNA and HMGA1 protein was detected in endometrial carcinoma cell lines AN3CA, ECC-1, Ishikawa and tissue specimens from cacinomatous endometrium （n=21） and normal endometrium （n=lS） by means of RT-PCR and Western blot analysis, respectively. [Results] The expression of HMGAI-mRNA and HMGA1 protein was detected in endometrial carcinoma cell lines AN3CA, ECC-1, Ishikawa. Higher expression of HMGA1 at both mRNA and protein level was detected in cacinomatous endometrium tissue, compared with normal endometrium tissue （P 〈0.05）. The expression level of HMGA1- mRNA and HMGA1 protein was correlated with clinical stages of endometrial carcinoma （P 〈0.05） and there was no significant differences in differentiated feature of endometrial carcinoma （P 〉0.05）. [Conclusions] The over-expression of HMGA1 may be involved in the development and tumorigenesis of endometrial carcinoma and maybe play an important role in cell proliferation and invasion of endometrial carcinoma.