高速逆流色谱法分离制备刺梨黄酮成分

Application of High Speed Countercurrent Chromatography to the Purification of Flavonoid Extract from Rosa roxburghii Tratt Fruit

应用高速逆流色谱法分离制备了刺梨中的黄酮类成分。以氯仿-甲醇-水(4:4:2,V/V)为两相溶剂系统,在主机转速为800r/min、流速1.0ml/min、检测波长254nm条件下进行分离制备,所得分离收集液经高效液相色谱法检测,结果表明,从刺梨黄酮粗提物中分离得到了纯度分别为75.6%、95.1%、73.5%、89.1%、87.7%的五种黄酮类成分;经干燥得对应样品重分别为21、8、11、20、55mg。该法具有简便、快速的优点。

Compared to traditional column chromatography, high speed countercurrent chromatography （HSCCC） has many advantages such as rapid separation, low sample adsorption, good separation reproducibility, etc. Therefore, this study employed HSCCC to purify crude flavonoid extract from Rosa roxburghii Tratt fruit. The purification was performed using a two- phase solvent system composed of chloroform, methanol and water （4：4：2, V/V） at a flow rate of 1.0 ml/min, when the rotation speed and the detection wavelength were set at 800 r/min and 254 nm, respectively. The results showed that five flavonoid compounds whose purities were 75.6%, 95.1%, 73.5%, 89.1% and 87.7%, respectively were obtained, and their masses were 21, 8, 11, 20 and 55 mg, respectively （obtained from 318 mg of dry crude flavonoid extract from Rosa roxburghii Tratt fruit）.