超临界CO_2反相微乳萃取人参皂甙的研究(英文)

Supercritical CO_2 Reverse Microemulsion Extraction of Ginsenosides from Panax ginseng

利用萃取罐体积为1L的超临界CO2萃取设备,采用琥珀酸二(2-乙基己基)酯磺酸钠(AOT)/乙醇/水/超临界CO2反相微乳对人参皂甙的萃取进行了研究。结果表明:最优萃取参数为萃取温度55℃,萃取时间3h,加水量36ml/100g人参,萃取压力30MPa和AOT添加量0.06mol/100g人参,此时人参皂甙的得率为0.757%;E=0.870×(1-e-0.618t)为超临界CO2反相微乳萃取人参皂甙的具体动力学模型方程;超临界CO2反相微乳萃取与超临界CO2萃取人参皂甙相比是一种相对有效的萃取技术。

The extraction of ginsenosides from Panax ginseng root using supercritical CO2 reverse microemulsion formed by bis（2-ethylhexyl） sodium sulfosuccinate （AOT）, ethanol and water was studied, which was carried out in a 1L supercritical CO2 extraction device. Single-factor and orthogonal array design methods were applied to optimize the extraction. The effects of 7 parameters on ginsenosides extraction were investigated by the single-factor method. A higher extraction yield of ginsenosides was achieved using ethanol as the cosufactant in comparison with butanol and n-pentanol, with the optimal added amount of 120 ml/100 g ginseng. Other 5 parameters such as amount of added water, amount of added AOT, extraction time, temperature and pressure were optimized using the orthogonal array design method. The optimal levels of the 5 parameters for improved ginsenosides extraction were determined as follows： amount of added water 36 ml/100 g ginseng, amount of added AOT 0.06 mol/100 g ginseng, extraction time 3 h, extraction temperature 55 ℃ and extraction pressure 30 MPa. An extraction yield of 0.757% was achieved under the optimized conditions. Moreover a dynamic model for ginsenosides yield as a function of extraction time was developed, which was E = 0.870×（1-e^-0.618t）. Compared to supercritical CO2 extraction （SCE）, this extraction method yielded more ginsenosides.