菱角壳总黄酮超声辅助提取工艺研究

Ultrasound-assisted Extraction of Total Flavonoids from Water Chestnut Hull

目的:确定菱角壳中总黄酮的优化超声回流提取工艺条件。方法:采用正交试验法考察溶剂用量、溶剂浓度、提取次数、提取时间对总黄酮提取率的影响,用标准曲线法测定总黄酮含量。结果:在超声提取所考察的因素中,采用50%的乙醇、取液料比10:1(V/m),超声提取2次、每次提取0.5h,在此条件下黄酮提取可达0.81%。结论:该方法具有操作简单、提取时间短、提取率高等优点。

Ultrasonic technique was employed to extract total flavonoids from water chestnut hull with ethanol. Effects of four crucial factors including ethanol concentration, liquid/material ratio （Win）, extraction duration and repeated extraction times on extraction yield of total flavonoids were investigated by one-factor-at-a-time method. Subsequent/y, all these factors were optimized using orthogonal array design. The optimal parameters for extracting total flavonoids were as follows： ethanol concentration 50% and liquid/material ratio 10：1 for an extraction duration of 0.5 h repeated twice, and the yield of total flavonoids was up to 0.81% under these optimized conditions. The ultrasonic-assisted extraction for total flavonoids in water chestnut hull is a time- and cost-saving and highly efficient method.