高效液相色谱荧光检测法测定虾中的4-己基间苯二酚

Determination of 4-Hexylresorcinol in Shrimp by HPLC with Fluorescence Detection

目的:建立快速检测虾中4-己基间苯二酚(4-HR)的高效液相色谱法。方法:用乙腈提取虾中的4-HR后,以反相C18柱为分离柱,以甲醇-水溶液为流动相洗脱,采用高效液相色谱-荧光检测器,于激发波长280nm,发射波长320nm定量检测。结果:4-HR在10min内出峰,线性范围为0.02～50.0mg/L(r=0.99996),检出限为0.1mg/kg,回收率为97.6%～99.9%,相对标准偏差(RSD)为2.1%～4.6%。结论:本法准确、可靠、简便、检出限低,适合分析大批量样品。

A rapid HPLC method was developed to determine 4-hexylresorcinol （4-HR） in shrimp meat. The sample was extracted with acetonitrile, filtrated through the 0.45 tam membrane and then injected into the HPLC column. The analysis was performed on a reversed-phase C18 column using a mobile phase consisting of methanol and water （70：30, V/V） with fluofirnetric detection （excitation： 280 nm, and emission： 320 nm）. The analyte could be separated within 10 min by the procedure. The linear range was 0.02-50.0 mg/L （r = 0.99996） and the detection limit was 0.1 mg/kg. The average recovery ranged from 97.6% to 99.9% （n = 7）, with a relative standard derivation from 2.1% to 4.6%. The method has the advantages of simplicity, repeatability and low detection limit, and can be used for analysis of shrimp samples in large quantity.