养阴活血汤早期干预对大鼠肺间质纤维化的影响

Treatment effect of decoction of Yangyin Huoxue Decoction on rats with pulmonary interstitial fibrosis in early stage

目的观察养阴活血汤对博莱霉紊诱导的大鼠肺间质纤维化疗效以及对转化生长因子β1（transforming growth factor-β1，TGF-β1）和结缔组织生长因子（connective tissue growth factor，CTGF）mRNA表达的影响。方法96只雌性Wistar大鼠，随机分为正常组、模型组、中药组和干扰索β1a（interferon-β1a，IFN-β1a）组。气管内注入博莱霉素A2建立大鼠肺间质纤维化模型。中药组自造模次日始，每只大鼠灌胃浓缩中药每天20ml／kg，IFN-β1a组皮下注射重组人IFN-β1a4．6μg／kg，每周3次。正常组和模型组等体积生理盐水灌胃。各组大鼠在实验周期第3天、第7天、第14天和第28天腹主动脉放血法处死，进行肺系数检测，HE和VG染色，原位杂交测定大鼠肺组织中TGF-β1和CTGF的mRNA表达情况。结果养阴活血汤能明显降低肺系数，使肺组织的病理改变减轻，可以减弱大鼠肺组织和原代培养肺成纤维细胞中TGF-β1mRNA和CTGFmRNA的表达强度。结论养阴活血汤可以减少TGF-β1mRNA和CTGFmRNA的表达量，减弱细胞因子对肺间质纤维化的促进作用，从而起到抑制肺问质纤维化的作用。

Objective To explore the treatment effect of Yangyin Huoxue Decoctior on pulmonary interstitial fibrosis in early stage and the mRNA expression of transforming growth factor-β1 （TGF-β1） and connective tissue growth factor（CTGF） in lung tissue and primary cultural fibroblasts. Methods Ninety-six female Wistar rats were randomly assigned into four groups：normal group, model group, traditional Chinese medicine（TCM） group and interferon-β1a（IFN-β1a） group. In each group,there were 24 rats. Models were founded by bleomycin A2. The rats in TCM group were treated with gastric perfusion of 20 ml/kg per day the decoction and IFN-β1a group were treated with IFN-β1a （4.6 μg/kg） through subcutaneous injection. The rats were killed at 3, 7, 14, 28 days of feeding, then the pulmonary coefficients, hematoxylin-eosin staining and Van Gieson Chan biter acid fuchinacid staining were done and the mRNA expressions of TGF-β1 and CTGF were detected by the nuclic acid by hybridization in situ technique. Resnlts Yangyin Huoxue Decoction decreased pulmonary coefficients significantly. It also weakened the level of fibrosis development of lung and the mRNA expression of TGF-β1 and CTGF in lung tissue. Conclusions Yangyin Huoxue Decoction can decrease the mRNA expression of TGF-β1 and CTGF in lung tissue. The formation of pulmonary interstitial fibrosis affected by the cytokines is weakened,in order to inhibit fibrotic development.