复方槐花颗粒质量标准研究

Quality Standard of Compound Huaihua Granules

目的：建立复方槐花颗粒的质量标准。方法：采用薄层色谱（TLC）法对方中槐花、丹参、金银花进行定性鉴别；采用紫外分光光度法测定槐花中芦丁的含量。结果：TLC斑点清晰、分离度好。芦丁的检测浓度在2．904-14．52μg·mL^-1范围内与峰面积积分值呈良好线性关系（r=0．9977）；平均加样回收率为98．43％，RSD=0．55％（n=6）。结论：所建标准可用于复方槐花颗粒的质量控制。

OBJECTIVE：To establish the quality standard for Compound huaihua granules.METHODS：Sophora japonica, salvia miltiorrhiza ,and Lonicera joponica in the formulation were identified qualitatively by TLC, and the content of rutin in Sophora japonica was determined by UV spectrophotometry. RESULTS： The TLC spots were clear and well - separated. The linear range of rutin was 2.904-14.52 μg· mL^-1（ r = 0.997 7） and its average recovery was 98.43% （RSD = 0.55%, n =6）. CONCLUSION： The established standard is suitable for the quality control of Compound huaihua granules.