RP-HPLC法测定养胃舒片中橙皮苷的含量

Determination of Hesperidin in Yangweishu Tablets by RP-HPLC

目的：建立以反相高效液相色谱法测定养胃舒片中橙皮苷含量的方法。方法：色谱柱为十八烷基硅烷键合硅胶柱（150mm×4．6mm，5μm），流动相为甲醇-7％醋酸水溶液（32：68），检测波长283nm。结果：橙皮苷进样量在49．6～892．8ng范围内与峰面积积分值呈良好线性关系（r=0．9999）；平均回收率为99．02％，RSD=0．45％（n=9）。结论：本方法简便、准确、重现性好，可用于养胃舒片的质量控制。

OBJECTIVE： To establish a RP-HPLC method for the determination of the content of hesperidin in Yangweishu tablets. METHODS： The samples were separated on octadecyl silane bonded gel silica column（ 150 mm × 4.6 mm, 5μm） with the mobile phase consisted of methanol - 7% acetic acid solution（32 ： 68） under a detection wavelength of 283nm. RESULTS： The linear range of hesperidin was 49.6-892.8 ng（ r = 0.999 9） with an average recovery rate of 99.02% （RSD = 0.45%, n = 9） .CONCLUSION： The method is simple, accurate, reproducible and applicable for the quality control of Yangweishu tablets.