SSR分子标记与水稻杂种优势的相关性研究

Study on the Correlation between SSR Molecular Markers and Rice Heterosis

[目的]探讨SSR标记遗传距离与水稻杂种优势的相关性。[方法]以5个不育系和4个恢复系为材料按5×4 NCⅡ方法配制20个杂交组合,选取36对与水稻产量相关的特异性SSR标记(与QTL连锁的标记)和124对非特异性SSR标记(一般标记)对9个供试亲本进行多态性分析,研究SSR标记遗传距离与水稻杂种优势的关系。[结果]特异性SSR标记遗传距离与水稻F1代各性状优势之间的相关性均不显著,而非特异性SSR标记遗传距离与株高、千粒重优势呈显著和极显著正相关,与结实率优势呈显著负相关,特异性SSR标记遗传距离与水稻单株产量优势相关更密切。[结论]SSR标记遗传距离与水稻杂种优势有一定的相关性,但相关系数较小,不足以预测水稻的杂种优势。

[ Objective ] The aim was to discuss the correlation between genetic distance of SSR markers and rice heterosis. [ Method] With 5 male sterile lines and 4 restorer lines as tested materials, 20 hybridized combinations were compounded on the basis of 5 × 4 NC Ⅱ method. Thirty-six pairs of specific SSR markers （markers linked to QTL） related to rice yield and 124 pairs of non-specific SSR markers （general markers） were selected for performing polymorphism analysis on 9 tested parents so as to study the correlation between genetic distance of SSR markers and rice heterosis. [ Result] The correlations between genetic distance of specific SSR markers and traits heterosis of rice generation F1 were not significant. BUt the genetic distance of non-specific SSR markers showed significant and extremely significant positive correlations with the heterosis in plant height and 1 000-grain weight, resp. and showed significandy negative correlation with seed setting rate. the genetic distance of specific SSR makers related to the heterosis of rice yield per palnt more closely. [ Conclusion ] There was a certain correlation between the genetic distance of SSR markers and rice heterosis, but their correlation coefficient was too small to predict rice heterosis.