低强度日光灯长期照射对大鼠视网膜的影响

Effect of low intensity artificial light on the retinal function of rats for a long time

目的:研究低强度日光灯长期循环照射对大鼠视网膜的影响。方法:SD大鼠30只随机分成两组,实验组模拟人类普通室内光环境,采用36W日光灯照明,明/暗交替时间为18/6h,对照组为自然光环境,实验时间4mo,观察大鼠眼电生理及视网膜组织学改变。结果:实验组Max-Ra,b波振幅下降54.3%,65.4%,Cone-Ra,b波振幅下降35.8%,38.5%,OpsOS值、30Hz Flicker N1-P1振幅下降,与对照组比较有显著性差异(P<0.05)。实验组整个视网膜普遍萎缩变薄,层次不清,对照组光镜下见视网膜各层分界清晰,排列整齐。对照组大鼠视网膜丙醛(malondialdehyde,MDA)含量低,超氧化物歧化酶(superoxidedismutase,SOD)活性高,与实验组比较差异有显著性(P<0.05)。日光灯频闪大,稳定性差,紫外区的辐射非常明显。结论:低强度人工光源日光灯长期照射影响大鼠视网膜功能。

AIM： To investigate the effect of low intensity artificial light on the retinal function of rats for a long time. METHODS： Thirty rats were divided into two groups randomly, The rats of the experimental group were exposed to 36W daylight lamp（ light： dark = 18： 6h） which imitated indoor light of human. The control group were exposed to natural light. After 4 months, we observed the retinal changs between two groups. RESULTS： The amplitude of a-wave and b-wave of Max-R in experimental group decreased by 54. 3% and 65.4% respectively. The amplitude of a-wave and b-wave of Cone-R decreased by 35.8% and 38.5% respectively. The amplitude of OS of Ops and N1-P1 of 30Hz Flicker decreased too. The amplitude of ERG in the experimental group were lower than that in the control group （ P〈 0.05）. The entire retina of the experimental group atrophied by optical microscope. The layers of retina of the experimental group were disorder. Retina was normal in the control group. The content of malondialdehyde（MDA） in control group was lower than that of experiment group（P〈 0. 057. The activity of superoxidedismutase （SOD） in control group was higher than that of experiment group（ P〈 0.05）. Daylight lamp brightness was not stable. Spectrum of daylight lamp contained much ultraviolet radiation. CONCLUSION： It suggested that low intensity artificial light can affect retinal function.