红色荧光蛋白标记的胰腺癌裸鼠原位移植瘤转移模型的建立

Establishment of red fluorescent protein orthotopic transplantation nude mice metastasis model of pancreatic cancer and whole-body fluorescent imaging

目的建立稳定、高表达红色荧光蛋白（RFP）标记的胰腺癌裸鼠原位移植瘤自发转移模型。方法将稳定、高表达RFP的人胰腺癌细胞株SW1990-RFP注射至裸鼠皮下，建立人胰腺癌裸鼠皮下移植瘤模型。通过外科手术原位移植裸鼠皮下荧光肿瘤组织小块，建立人胰腺癌裸鼠原位移植瘤自发转移模型。应用整体荧光成像系统评价人胰腺癌裸鼠原位移植瘤模型肿瘤转移及微转移的发生情况。结果成功建立12只人胰腺癌裸鼠原位移植瘤自发转移模型，建模成功率为100％。应用整体荧光成像系统可以实时监测原发肿瘤的生长状况以及在肿瘤生长早期即可检测到各脏器微小转移的发生。结论本实验所建立的RFP标记的胰腺癌裸鼠原位移植瘤自发转移模型稳定、可靠，可以在体外无创性动态观察肿瘤的发生、发展，具较强的灵敏性及特异性。

Objective To establish a stable high red fluorescent protein （RFP）-expressing orthotopic transplantation nude mice spontaneous metastasis model of pancreatic cancer. Methods Stable high RFP-expressing cells SW1990-RFP were injected subcutaneously into mice to establish subcutaneous implantation model. Fluorescent tumor piece from subcutaneous was transplanted into the body of the pancreas to establish surgical orthotopic implantation model. The growth of primary tumor, metastasis and micrometastasis were assessed by whole-body fluorescence imaging system. Results Twelve RFP orthotopic transplantation nude mice metastasis models of pancreatic cancer were established suceeeddly, the percentage of success rate was 100%. RFP-labeled pancreatic cancer growth could be monitored in real time way. The micrometastasis of primarylesions were detected in early stage with whole-body fluorescence imaging system. Conclusions The RFP orthotopic transplantation nude mice metastasis model of pancreatic cancer is stable and reliable, and can be observed dynamically in vitro in a noninvasive way, with much higher sensitivity and specificity.